Phytochemical and antibacterial screening of different fractions of root part of Ipomea Turpethum
Keywords:Ipomea turpethu, , phytochemical screening, antibacterial activity
Objective: In folk medicines, different herbs and plants have been used for many thousands of years .Now it is important to investigate these plants and herbs scientifically which have been used in traditional medicines. The aim of this study was to investigate the antibacterial activity and preliminary phytochemical screening of root of Ipomea turpethum extracted in methanol and its fractions.
Methods: The methanol extract was further extracted with three solvents ethyl acetate, chloroform and hexane and analyzed for their antibacterial activity using by agar well diffusion method. They were tested against six bacteria; Echrichia coli, Staphylococcus aureus, Staphylococcus epidermidus, Proteus vulgaris, Pseudomonas auroginosa and Salmonella typhi. The susceptibility of microorganisms to all three fractions was compared with each other and with standard antibotic (Ampicillin). The fractions of Ipomea turpethum was also qualitatively analyzed for the presence of chemical components, i.e. saponins, alkaloids, flavonoids, tannins and glycosides.
Result: Among all fractions methanol exhibited highest antibacterial activity (average zone of inhibition 23.53mm ± 1.3) while ethyl acetate exhibited least antibacterial activity (average zone of inhibition 18.50mm ± 3.5). Minimum inhibitory concentration of methanol, ethyl acetate, chloroform and hexane fractions was found in the range of 650ug/ml to 2500ugl/ml against microorganisms.
Conclusion: Results obtained from this preliminary in-vitro experiment indicate that, all three fractions of Ipomea turpethum has good antibacterial activity against all microorganisms used. By phytochemical analysis of extract, it has been found to contain some nutrient and chemical components which support its ethenomedicinal use but further work is required for development of new antibiotic compounds.
Bangladesh Journal of Medical Science Vol.17(1) 2018 p.93-97
How to Cite
Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:
- Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.
Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.