Effect of Secretome Mesenchymal Stem Cells On Expression Interleukin 10 And Interleukin 17 in Mice Lupus Model

Authors

  • Arif Nurudhin Sp.PD, FINASIM, Perum Flamboyan Indah No IA, Blulukan, Colomadu, Karanganyar and Doctoral Program of Medical Sciences Faculty of Medicine Sebelas Maret University Indonesia
  • Zainal Arifin Adnan Sp.PD,KR,FINASIM, Division of Rheumatology, Department of Internal Medicine, Dr. Moewardi General Hospital, Faculty of Medicine, Sebelas Maret University, Surakarta
  • Nyoman Kertia Sp.PD,KR,FINASIM, Division of Rheumatology, Department of Internal Medicine, Dr. Sardjito General Hospital, Faculty of Medicine, Gadjah Mada University, Yogyakarta

DOI:

https://doi.org/10.3329/bjms.v16i3.32862

Keywords:

Interleukin 10, Interleukin 17, Pristane, Secretome mesenchymal stem cell, Systemic Lupus Erythematosus

Abstract

Background: Systemic Lupus Erythematosus (SLE) is a chronic autoimmune inflammatory disease with extensive clinical features. The role of Interleukin 10 (IL-10) is to promote autoantibody production and autoreactive B cell proliferation. Interleukin 17 (IL-17) associated with pathogenesis of SLE and positively correlated with disease activity. Intraperitoneal pristane can induce lupus in mice. Mesenchymal stem cells secretome work in paracrine effects asantiinflammatory and immunomodulation agent by suppressing autoreactive T and B cell, which play an important role in pathogenesis of SLE. The aim of this study is to evaluate effect of mesenchymal stem cells secretome on the expression of IL-10 and IL-17 in murine lupus models induced by pristane.

Methods: A randomized experimental study, post-test only control group design, samples using 21 female Mus Musculus mice strain Balb/C, divided into 3 groups: control group with intraperitoneal injection of 0.5 mL of 0.9% NaCl, treatment group with intraperitoneal injection of 0.5 mL pristane, therapy group with intraperitoneal injection of 0.5 mL pristane and 0.45 mL of secretome. Statistical analysis using ANOVA test. A p-value < 0.05 was considered statistically significant.

Results: The results showed significant relationship between control and pristane groups both at the levels of IL-17 (control 6.9±1.95,pristane 9.9±2.27, pristane+secretome 6.1±1.95 p=0.016), and there are significant differences in the expression of IL-10 in the control group vs pristane group (-4,42±1,43 per 100 lymphocyte; p=0,006), pristane group vs pristane+secretome group (4,00±1,43 per 100 lymphocyte; p=0,012).

Conclusion: Mesenchymal stem cells secretome decreased the expression of IL-10 and IL-17 levels in murine lupus models induced by pristane.

Bangladesh Journal of Medical Science Vol.16(3) 2017 p.418-422

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Author Biography

Arif Nurudhin, Sp.PD, FINASIM, Perum Flamboyan Indah No IA, Blulukan, Colomadu, Karanganyar and Doctoral Program of Medical Sciences Faculty of Medicine Sebelas Maret University Indonesia



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Published

2017-06-09

How to Cite

Nurudhin, A., Adnan, Z. A., & Kertia, N. (2017). Effect of Secretome Mesenchymal Stem Cells On Expression Interleukin 10 And Interleukin 17 in Mice Lupus Model. Bangladesh Journal of Medical Science, 16(3), 418–422. https://doi.org/10.3329/bjms.v16i3.32862

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Original Articles