Cloning of Multidrug-Resistant Genes from <i>Vibrio cholerae</i> Non-O1 NCTC4716
Keywords:Vibrio cholerae, Drug resistance, Drug-resistant genes, Cloning, Minimum inhibitory concentration (MIC)
Both clinical and environmental multidrug-resistant strains of Vibrio cholerae (O1 and non-O1) have been reported from different parts of the world. In this study, we have cloned twenty-five genes associated drug resistance in Vibrio cholerae non-O1 NCTC4716 using shotgun cloning method. All of the cloned genes were introduced and expressed in a drug hypersusceptible host Escherichia coli KAM32. We observed elevated minimum inhibitory concentration (MIC) of various antimicrobial agents such as fluoroquinolones, aminoglycosides, ethidium bromide and others in all transformants.
Keywords: Vibrio cholerae, Drug resistance, Drug-resistant genes, Cloning, Minimum inhibitory concentration (MIC)
DOI: http://dx.doi.org/10.3329/bjm.v23i2.879Bangladesh J Microbiol, Volume 23, Number 2, December 2006, pp 137-139