Cloning of Multidrug-Resistant Genes from <i>Vibrio cholerae</i> Non-O1 NCTC4716

Authors

  • Anowara Begum Department of Molecular Microbiology, Graduate School of Medicine, Dentistry & Pharmaceutical Sciences, Okayama University, Okayama 700 8530, Japan
  • Teruo Kuroda Department of Molecular Microbiology, Graduate School of Medicine, Dentistry & Pharmaceutical Sciences, Okayama University, Okayama 700 8530, Japan
  • Tomofusa Tsuchiya Department of Molecular Microbiology, Graduate School of Medicine, Dentistry & Pharmaceutical Sciences, Okayama University, Okayama 700 8530, Japan

DOI:

https://doi.org/10.3329/bjm.v23i2.879

Keywords:

Vibrio cholerae, Drug resistance, Drug-resistant genes, Cloning, Minimum inhibitory concentration (MIC)

Abstract

Both clinical and environmental multidrug-resistant strains of Vibrio cholerae (O1 and non-O1) have been reported from different parts of the world. In this study, we have cloned twenty-five genes associated drug resistance in Vibrio cholerae non-O1 NCTC4716 using shotgun cloning method. All of the cloned genes were introduced and expressed in a drug hypersusceptible host Escherichia coli KAM32. We observed elevated minimum inhibitory concentration (MIC) of various antimicrobial agents such as fluoroquinolones, aminoglycosides, ethidium bromide and others in all transformants.

Keywords: Vibrio cholerae, Drug resistance, Drug-resistant genes, Cloning, Minimum inhibitory concentration (MIC)

DOI: http://dx.doi.org/10.3329/bjm.v23i2.879

Bangladesh J Microbiol, Volume 23, Number 2, December 2006, pp 137-139

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How to Cite

Begum, A., Kuroda, T., & Tsuchiya, T. (2008). Cloning of Multidrug-Resistant Genes from <i>Vibrio cholerae</i> Non-O1 NCTC4716. Bangladesh Journal of Microbiology, 23(2), 137–139. https://doi.org/10.3329/bjm.v23i2.879

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