Effect of the combined use of Esomeprazole and Imipenem against Pseudomonas aeruginosa

Authors

  • Tariqul Islam Stamford University, Department of Microbiology, Dhaka
  • Abdullah Al Mamun Stamford University, Department of Microbiology, Dhaka
  • Debashish Sarker Stamford University, Department of Microbiology, Dhaka
  • Sunjukta Ahsan Department of Microbiology, University of Dhaka, Dhaka
  • Md Shahidul Kabir Stamford University, Department of Microbiology, Dhaka

Keywords:

Antimicrobial activity, Biofilm, Biomass, Bacterial infection

Abstract

Antimicrobial activity of esomeprazole (0.25 mM) and imipenem (30 μg/ml) was tested independently and in combination against planktonic and biofilm associated cells of Pseudomonas aeruginosa. Esomeprazole (0.25 mM) and imipenem (30 μg/ml) were added to broth media in test tubes to determine their effect on growth of Pseudomonas aeruginosa at 2, 4, 6, 24 and 72 hours after inoculation at 37°C. Viable bacterial counts (cfu/ml) were measured by dilution and plating on nutrient agar medium and the biomass was measured at 600 nm using a spectrophotometer. Biomasses of bacterial biofilms were determined by tube adherence assay and measuring the absorbance at 580 nm wavelength. Pseudomonas aeruginosa showed decreased growth and biomass of sessile bacteria (p<0.05) after exposure to imipenem and esomeprazole compared to untreated controls after 48 and 72 hours of exposureat 37°C. However, the reduction in viable cells after exposure to esomeprazole was not significant after 48 and 72 hours of incubation at 37°C (p>0.05). Biomass of bacterial biofilms were significantly reduced after exposure to esomeprazole and imipenem compared to untreated controls (p<0.05) as determined by tube adherence assay. Esomeprazole and imipenem demonstrated antibacterial and anti-biofilm property against planktonic and biofilm embedded cells of P. aeruginosa which may be useful for treatment of bacterial infections.

Bangladesh J Microbiol, Volume 34 Number 1 June 2017, pp 21-25

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How to Cite

Islam, T., Mamun, A. A., Sarker, D., Ahsan, S., & Kabir, M. S. (2018). Effect of the combined use of Esomeprazole and Imipenem against Pseudomonas aeruginosa. Bangladesh Journal of Microbiology, 34(1), 21–25. Retrieved from https://www.banglajol.info/index.php/BJM/article/view/39227

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