Virulence Factors and Molecular cloning of Outer Membrane Protein (OMP) gene from virulent Aeromonas hyrophila isolated from infected gold fish Carassius auratus

Authors

  • V Thanga Viji Centre for Marine Science and Technology, Manonmaniam Sundaranar University Rajakkamangalam, Kanyakumari District, Tamilnadu
  • M Michael Babu Centre for Marine Science and Technology, Manonmaniam Sundaranar University Rajakkamangalam, Kanyakumari District, Tamilnadu
  • S Velmurugan Centre for Marine Science and Technology, Manonmaniam Sundaranar University Rajakkamangalam, Kanyakumari District, Tamilnadu
  • T Kumaran Centre for Marine Science and Technology, Manonmaniam Sundaranar University Rajakkamangalam, Kanyakumari District, Tamilnadu
  • S B Anand NRCBS, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamilnadu
  • P Gunasekaran NRCBS, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamilnadu
  • T Citarasu Centre for Marine Science and Technology, Manonmaniam Sundaranar University Rajakkamangalam, Kanyakumari District, Tamilnadu

DOI:

https://doi.org/10.3329/bjm.v28i2.11819

Keywords:

Aeromonas hyrophila, Outer Membrane Protein, Carassius auratus, Cloning

Abstract

Aeromonas hyrophila strains AHV1, AHV2 and AH3 were isolated and identified from Muscle tissue, intestine, body fluid and gills of infected gold fish Carassius auratus. In order to study their virulence, LD50 tests against  normal gold fish, proteolytic, haemolytic and challenge studies were performed. The virulence studies revealed that, both AHV1 and AHV2 strains are highly positive for proteolytic and haemolytic properties. The LD50 data showed that, the fish C. auratus are highly susceptible to A. hyrophila strains AHV1 and AHV2 at cent percent lethal rate. The survival of C. auratus significantly (P<0.05) decreased when challenged with virulent strains of A. hydrophila AHV1 and AHV2. The outer membrane protein (OMP-TS) gene was successfully amplified generating an the amplicon size of 1008 bp. The amplified product from the genomic DNA of AHV1 strain was cloned in to pTZ57R/T vector, transformed into DH5? cells and sequenced. The sequenced clone is resembling to various A. hydrophila isolates and submitted to NCBI GenBank database (accession no:HQ331525).

DOI: http://dx.doi.org/10.3329/bjm.v28i2.11819

Bangladesh J Microbiol, Volume 28, Number 2, December 2011, pp 70-75

Downloads

Download data is not yet available.
Abstract
2708
PDF
967

Downloads

Published

2012-09-05

How to Cite

Viji, V. T., Babu, M. M., Velmurugan, S., Kumaran, T., Anand, S. B., Gunasekaran, P., & Citarasu, T. (2012). Virulence Factors and Molecular cloning of Outer Membrane Protein (OMP) gene from virulent Aeromonas hyrophila isolated from infected gold fish Carassius auratus. Bangladesh Journal of Microbiology, 28(2), 70–75. https://doi.org/10.3329/bjm.v28i2.11819

Issue

Section

Articles