Antioxidant, thrombolytic, cytotoxic and membrane stabilizing activities and fatty acid composition of Acmella Calva (DC.) Flower

Authors

  • Md Omar Sha Rafi Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh
  • Nusrat Tabassum Shristy Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh
  • Sauda Sultana Mimi Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh
  • Sadia Afrin Chhanda Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh
  • Md Hasanur Rahman Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh
  • Mohammad Sharifur Rahman Department of Pharmaceutical Chemistry, University of Dhaka, Dhaka-1000, Bangladesh.
  • Mohammad A Rashid Department of Pharmaceutical Chemistry, University of Dhaka, Dhaka-1000, Bangladesh.
  • Tanvir Muslim Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh

DOI:

https://doi.org/10.3329/bjb.v25i3.84427

Keywords:

Acmella calva, Antioxidant, Thrombolytic Activity, Cytotoxicity, Membrane Stabilizing Activity

Abstract

In the present study, crude methanolic extract (CRME) of A. calva flower along with its n-hexane (HSF), ethyl acetate (EASF), dichloromethane (DCMSF) and aqueous (AQSF) soluble fractions were subjected to antioxidant, thrombolytic, cytotoxic and membrane stabilizing activities. Fatty acid composition of the n-hexane extract was analysed by gas chromatography-flame ionization detector. None of the extracts showed any cytotoxicity against both carcinoma (HeLa) and normal cell lines (Vero and BHK-21). EASF scavenged the DPPH free radical most actively with IC50 value of 35.05 μg/ml. CRME and HSF showed profound membrane stabilizing activity by protecting human erythrocyte cell against hypotonic solution-induced and heat-induced lysis. CRME showed the highest (46%) clot lysis. Stearic acid (51.11%) and palmitoleic acid (39.38%) were found in the highest amount in both bound and free form of fatty acid, respectively. The observation from this study indicates the possibility of A. calva to be a good source of different bioactive phytochemicals.

Bangladesh J. Bot. 54(3): 433-439, 2025 (September)

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Published

2025-09-29

How to Cite

Rafi, M. O. S., Shristy, N. T., Mimi, S. S., Chhanda, S. A., Rahman, M. H., Rahman, M. S., … Muslim, T. (2025). Antioxidant, thrombolytic, cytotoxic and membrane stabilizing activities and fatty acid composition of Acmella Calva (DC.) Flower . Bangladesh Journal of Botany, 54(3), 433–439. https://doi.org/10.3329/bjb.v25i3.84427

Issue

Vol. 54 No. 3 (2025)

Section

Articles

License

© Bangladesh Botanical Society

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