In Vitro Regeneration of Aromatic Rice (Oryza Sativa L. Var. Doairgura)

Authors

  • Barna Goswami Bangladesh Council of Scientific and Industrial Research, Dhamondi, Dhaka-1205, Bangladesh
  • Tanjina Akhtar Banu Bangladesh Council of Scientific and Industrial Research, Dhamondi, Dhaka-1205, Bangladesh
  • Shahina Akter Bangladesh Council of Scientific and Industrial Research, Dhamondi, Dhaka-1205, Bangladesh
  • Sadia Afrin Bangladesh Council of Scientific and Industrial Research, Dhamondi, Dhaka-1205, Bangladesh
  • Ahashan Habib Bangladesh Council of Scientific and Industrial Research, Dhamondi, Dhaka-1205, Bangladesh
  • Salim Khan Bangladesh Council of Scientific and Industrial Research, Dhamondi, Dhaka-1205, Bangladesh

DOI:

https://doi.org/10.3329/bjb.v51i4.63485

Keywords:

In vitro regeneration, Aromatic rice, Doairgura

Abstract

Rice (Oryza sativa L.) is the world’s most important cereal crop. The Doairgura variety (O. sativa L.) is a low-yielding, high-quality aromatic rice-producing variety in Bangladesh. This study was undertaken to develop an in vitro regeneration protocol through in vitro callus induction. MS media supplemented with different concentrations of 2, 4- D (0.5, 1.0, 2.0 and 3.0 mg /l) were used for callus induction from the mature dehusked rice seeds. The best results for callus formation was obtained on MS media supplemented with 2.0 mg/l 2, 4-D. Shoot regeneration from in vitro cultured callus was tested with different combinations (BAP, NAA, Kn mg/l) of hormone containing MS media. The highest regeneration frequencies (100%) as well as the highest average shoot per culture (15.3) were recorded on the MS media supplemented with 2.0 mg/l BAP and 1.0 mg/l NAA. For root induction, hormone free MS media showed better results. After hardening, the plantlets were transferred to soil. This protocol is relatively simple and reproducible.

Bangladesh J. Bot. 51(4): 677-682, 2022 (December)

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Published

2022-12-29

How to Cite

Goswami, B. ., Banu, T. A. ., Akter, S. ., Afrin, S. ., Habib , A. ., & Khan, S. . (2022). In Vitro Regeneration of Aromatic Rice (Oryza Sativa L. Var. Doairgura). Bangladesh Journal of Botany, 51(4), 677–682. https://doi.org/10.3329/bjb.v51i4.63485

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