Regeneration Efficiency Of Five High Yielding Potato Varieties Of Bangladesh
Keywords:Potato, Regeneration, Explant, Internode, Leaf, Zeatin riboside
Experiments were conducted to develop an efficient and reliable protocol for in vitro direct regeneration of potato for future transformation. Five varieties of potato viz. BARI Alu-7 (Diamant), BARI Alu-21 (Provento), BARI Alu-24 (Dura), BARI Alu-25 (Asterix) and BARI Alu-26 (Felsina); 3 levels of Zeatin riboside viz. 3, 4 and 5 mg/l ; 2 types of explants viz. internode and leaf from 19-22 days old micro plants were used in this study. Internode explants of BARI Alu-25 produced the highest number of shoots (40.17) within16 days without callus phase in MS medium supplemented with 5 mg/l ZR + 0.2 mg/l GA3 + 0.01 mg/l IAA followed by BARI Alu-21 (38.13), BARI Alu-7 (37.49), respectively. Leaf explants also produced shoots directly without callus phase in the same medium. In this case, the highest number (7.87) of shoots was found from the variety BARI Alu-25 followed by BARI Alu-21(7.42). ½MS medium supplemented with 0.5 – 1.0mg/l IBA resulted the best performance as to the rooting from ZR treated regenerated shoots having 100% plant survival at ex vitro conditions. This protocol may be used for rapid shoot proliferation and genetic transformation in potato.
Bangladesh J. Bot. 51(4): 637-645, 2022 (December)
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