RAPD analysis of Asplenium adulterinum Milde from in vitro culture

Authors

  • Catană Rodica Institute of Biology Bucharest, Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, PO Box 56-53, Romania
  • Aldea Florentina Institute of Biology Bucharest, Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, PO Box 56-53, Romania
  • Paica Ioana Institute of Biology Bucharest, Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, PO Box 56-53, Romania

DOI:

https://doi.org/10.3329/bjb.v51i2.60437

Keywords:

Critically endangered, Fern, Glaciar relict, Genetic similarity, Tissue culture

Abstract

Asplenium adulterinum Milde represents one of the natural heritages which have to be protected due to their scientific importance. In the actual context of global warming with myriad effects on biodiversity, the ex-situ conservation of A. adulterinum should be a priority. The possibility to obtain genetically stable individuals through in vitro techniques is a requirement of ex situ conservation. The genetic similarity of A. adulterinum gametophytes maintained 8 years in vitro culture was assessed. Explants represented by gametophyte fragments were analyzed for genetic stability through RAPD. Close similarity (88.88%) was found between gametophytes maintained in the same conditions (medium, illumination, temperature, passages number).

Bangladesh J. Bot. 51(2): 387-391, 2022 (June)

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Published

2022-06-28

How to Cite

Rodica, C. ., Florentina , A. ., & Ioana, P. . (2022). RAPD analysis of Asplenium adulterinum Milde from in vitro culture. Bangladesh Journal of Botany, 51(2), 387–391. https://doi.org/10.3329/bjb.v51i2.60437

Issue

Section

Short Communications