Production and Optimization of Cellulase Activity of Thermomonospora Viridis Isolated From Rice Straw

Authors

  • Faozia Faleha Sadida Institute of Education and Research, Faculty of Arts and Humanities, University of Chittagong, Chittagong 4331, Bangladesh
  • Ma Manchur Department of Microbiology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh

DOI:

https://doi.org/10.3329/bjb.v50i2.54097

Keywords:

Rice straw, Cellulases, CMCase, Winstead’s medium, Thermomonospora viridis

Abstract

A highly cellulolytic actinomycete SR1 was locally isolated from rice straw and provisionally identified as Thermomonospora viridis. Optimum pH, temperature, carbon and nitrogen sources for its cellulase production were 6.5, 35°C, Carboxymethyl cellulase (CMC) and yeast extract, respectively whereas those of cellulase activity were 7.5, 40°C, CMC and peptone respectively. The effects of various metal ions and different reductant and inhibitors on its cellulase activity were investigated. Univalent Ag+ was found to decrease the enzyme activity whereas increased by bivalent Mg2+. Ethylene diamine tetraacetic acid (EDTA) caused remarkable decrease of cellulase activity but β-Mercaptoethanol stimulated its cellulase activity.

Bangladesh J. Bot. 50(2): 395-404, 2021 (June)

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Published

2021-06-18

How to Cite

Sadida, F. F., & Manchur, M. (2021). Production and Optimization of Cellulase Activity of Thermomonospora Viridis Isolated From Rice Straw. Bangladesh Journal of Botany, 50(2), 395–404. https://doi.org/10.3329/bjb.v50i2.54097

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Section

Articles