Indirect organogenesis and somatic embryogenesis for regeneration of Rauvolfia serpentina L. from root explants

Authors

  • Tanjina Akhtar Banu Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Salim Khan Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Barna Goswami Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Sadia Afrin Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Ahashan Habib Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Shahina Akter Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

DOI:

https://doi.org/10.3329/bjb.v49i4.52534

Keywords:

Rauvolfia serpentina, Somatic embryogenesis, Indirect organogenesis

Abstract

Highest frequencies (75%) of friable embryogenic nodular calli were recorded from the root explants in the presence of MS + 4.0 mg/l BAP and 4.0 mg/l NAA. In combinations of BAP and NAA 45.4% callus produced direct plantlets formation and 36.3% only from aerial part of shoots. MS medium with 1.0 mg/l BAP and 0.5 mg/l Kn produced compact callus where 13.3% produced direct plantlets formation and 45.7% produced only shoot formation. Well-developed globular somatic embryos were found to form when the callus were cultured more than 6 - 8 weeks on MS with 1.0 mg/l BAP and 0.5 mg/l Kn. After acclimatization the well rooted plantlets were transferred to pot and grown successfully in soil.

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Published

2020-12-31

How to Cite

Banu, T. A., Khan, S., Goswami, B., Afrin, S., Habib, A., & Akter, S. (2020). Indirect organogenesis and somatic embryogenesis for regeneration of Rauvolfia serpentina L. from root explants. Bangladesh Journal of Botany, 49(4), 1021–1027. https://doi.org/10.3329/bjb.v49i4.52534

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