Phylogenetic study of living fossil Psilotum nudum L. from Himalayan range of Pakistan using DNA barcodes

Authors

  • Bushra Khan Department of Genetics, Hazara University Mansehra, 21300, Pakistan
  • Khushi Muhammad Department of Genetics, Hazara University Mansehra, 21300, Pakistan
  • Zainab Hamayun Islamia College, Peshawar, KP-Pakistan
  • Shafee Ur Rehman Islamia College, Peshawar, KP-Pakistan
  • Abdul Majid Department of Botany, Hazara University Mansehra, Pakistan-21300
  • Youxiong Que Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China
  • Muhammad Shahid Nadeem Department of Biochemistry, Faculty of Science, King Abdulaziz University Jeddah 21589, Saudi Arabia
  • Imdad Kaleem Department of Bioscience, COMSATS University, Islamabad, Pakistan

DOI:

https://doi.org/10.3329/bjb.v48i3.47725

Keywords:

Phylogenetic, Psilotum nudum, DNA barcodes

Abstract

The genus Psilotum belongs to Psilotaceae family, with about 133 to 189 species having outstanding social, economic and medically important relict plants. However, being living fossil plant, many hurdles come in way while trying to discern its phylogenetics reconstruction. The partial conserve nucleotide sequences of rbcLa, trnA, trnV, matK, ITS, ycF3 and rpoB genes were used to select the best suitable barcodes in Psilotum nudum L. The amplified fragments were subjected to Sanger sequencing and then Maximum likelihood, Maximum Parsimony and Neighbor Joining trees were generated using bioinformatics software. i.e., MUSCLE, BioEdit and Mega-7. Current population showed the best match between P. complanatum (KY099851.1), population reported from New Zealand and P. nudum (F384430.1) with identity value 100 and 99% having E-value 0.0 and 0.0 by rbcLa and trnA barcode region, respectively. The findings showed that P. nudum L. sequences are ever first reported from the Himalaya regions of Pakistan and could be utilized for further comparison among different populations of this relict plant species across the globe.

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Published

2019-09-30

How to Cite

Khan, B., Muhammad, K., Hamayun, Z., Ur Rehman, S., Majid, A., Que, Y., Nadeem, M. S., & Kaleem, I. (2019). Phylogenetic study of living fossil Psilotum nudum L. from Himalayan range of Pakistan using DNA barcodes. Bangladesh Journal of Botany, 48(3), 457–466. https://doi.org/10.3329/bjb.v48i3.47725

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Articles