Assay of xylanolytic activity of mutants of Neurospora crassa (Ema 5297) induced with ultra-violet radiation
Keywords:Mutation, Mutants, Xylanase enzyme, Enzymatic activity
Mutation was induced in Neurospora crassa (Ema 5297) with UV (having the wave length of 254 nm) irradiation. Three groups of biochemical mutants (leucine, tryptophan and arginine) were obtained. Ema and 7 leucine mutants were used to evaluate whether mutation has any considerable effect for the production of such enzyme. Enzymatic activity was expressed as International Unit (IU). It was found that N. crassa mycelia secrete xylanases to the culture supernatant. Mutant Y10 showed highest xylanase activity (1.663 IU) and wild N. crassa (Ema) showed lowest (0.416 IU) activity.
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