Factors affecting Agrobacterium mediated transformation of indigenous Chlorella vulgaris Bayerinck

Authors

  • Nadia Sharif Department of Biotechnology, Lahore College for Women University, Lahore
  • Neelma Munir Department of Biotechnology, Lahore College for Women University, Lahore
  • Faiza Saleem Department of Biotechnology, Lahore College for Women University, Lahore
  • Shugufta Naz Department of Biotechnology, Lahore College for Women University, Lahore

DOI:

https://doi.org/10.3329/bjb.v44i2.38524

Keywords:

Acetosyringone, Transformation, Chlorella vulgaris

Abstract

Chlorella vulgarisis Bayerinck widely used as a health food, feed supplement, as well as in the pharmaceutical, biofuel and cosmetics industries. It has been used to determine optimum transformation conditions through Agrobacterium tumefaciens. It has been revealed that bacterial density of OD600 = 1.0, 3 days of co-cultivation at 25°C in pH 5.5, and 100 μM acetosyringone are the optimum conditions to transform C. vulgaris.

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Published

2018-10-13

How to Cite

Sharif, N., Munir, N., Saleem, F., & Naz, S. (2018). Factors affecting Agrobacterium mediated transformation of indigenous Chlorella vulgaris Bayerinck. Bangladesh Journal of Botany, 44(2), 323–326. https://doi.org/10.3329/bjb.v44i2.38524

Issue

Section

Short Communications