Effects of carbon and nitrogen sources on production of proteases by Bacillus subtilis IC-5

Authors

  • Sereen Gul PCSIR Laboratories, P.O. Box 387, Mastung Road, Quetta, Balochistan
  • Mujeeb Ur Rahman Department of Botany, University of Balochistan, Quetta, Balochistan
  • Mohammad Ajmal PCSIR Laboratories, P.O. Box 387, Mastung Road, Quetta, Balochistan
  • Abdul Kabir Khan Achakzai PCSIR Laboratories, P.O. Box 387, Mastung Road, Quetta, Balochistan
  • Asim Iqbal Department of Zoology, University of Balochistan, Quetta, Balochistan

DOI:

https://doi.org/10.3329/bjb.v44i2.38518

Keywords:

Carbon, Nitrogen, Production, Proteases, Bacillus subtilis

Abstract

The effects of various carbon and nitrogen sources were evaluated on production of proteases by Bacillus subtilis IC-5. Both type and concentration of carbon and nitrogen sources influenced the production of proteases. Among the carbon sources glucose was found to be the most effective. It gave maximum production at 2% w/v concentration i.e., 1875 and 950 U/ml, alkaline and neutral protease, respectively. The response of Bacillus subtilis IC-5 towards synthesis and excretion of enzymes varied with the type of nitrogen sources. The addition of organic nitrogen sources to basal medium repressed the synthesis of proteases while the addition of inorganic nitrogen source such as sodium nitrate was found to be the best stimulating for alkaline and neutral protease synthesis. Sodium nitrate enhanced the production up to 62.40 and 10.52% of alkaline and neutral protease, respectively against w.r.t. control.

Downloads

Download data is not yet available.
Abstract
678
PDF
956

Downloads

Published

2018-10-13

How to Cite

Gul, S., Rahman, M. U., Ajmal, M., Achakzai, A. K. K., & Iqbal, A. (2018). Effects of carbon and nitrogen sources on production of proteases by Bacillus subtilis IC-5. Bangladesh Journal of Botany, 44(2), 285–292. https://doi.org/10.3329/bjb.v44i2.38518

Issue

Section

Articles