Assessment of genetic and epigenetic variations in potato somatic hybrids by Methylation-sensitive ISSR and RAPD markers

Authors

  • Jagesh Kumar Tiwari Central Potato Research Institute, Shimla, Himachal Pradesh 171 001
  • Shaumaya Saurabh Central Potato Research Institute, Shimla, Himachal Pradesh 171 001
  • Poonam Chandel Central Potato Research Institute, Shimla, Himachal Pradesh 171 001
  • Bir Pal Singh Central Potato Research Institute, Shimla, Himachal Pradesh 171 001
  • Vinay Bhardwaj Central Potato Research Institute, Shimla, Himachal Pradesh 171 001

DOI:

https://doi.org/10.3329/bjb.v44i1.22722

Keywords:

DNA methylation, In vitro propagation, Potato, Somatic hybrids

Abstract

Genetic and epigenetic changes (DNA methylation) in the mother plants of somatic hybrids (1st cycle) and their regenerants (30th cycles sub-cultured) were analyzed using methylation-sensitive ISSR and RAPD markers. ISSR and RAPD profiles and their cluster analyses based on the Jaccards similarity coefficient revealed 100% genetic similarity among the mother plant and their regenerants. While for the epigenetic changes in the samples, the MS-ISSR markers detected DNA methylation (2.7%) in the regenerants compared to the mother plant (1%), whereas MS-RAPD markers detected a little methylation patterns in the regeneratns (0.6%) compared to the mother plants (0.4%).

DOI: http://dx.doi.org/10.3329/bjb.v44i1.22722

Bangladesh J. Bot. 44(1): 45-50, 2015 (March)

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Published

2015-03-26

How to Cite

Tiwari, J. K., Saurabh, S., Chandel, P., Singh, B. P., & Bhardwaj, V. (2015). Assessment of genetic and epigenetic variations in potato somatic hybrids by Methylation-sensitive ISSR and RAPD markers. Bangladesh Journal of Botany, 44(1), 45–50. https://doi.org/10.3329/bjb.v44i1.22722

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