Assessment of genetic and epigenetic variations in potato somatic hybrids by Methylation-sensitive ISSR and RAPD markers
Keywords:DNA methylation, In vitro propagation, Potato, Somatic hybrids
Genetic and epigenetic changes (DNA methylation) in the mother plants of somatic hybrids (1st cycle) and their regenerants (30th cycles sub-cultured) were analyzed using methylation-sensitive ISSR and RAPD markers. ISSR and RAPD profiles and their cluster analyses based on the Jaccards similarity coefficient revealed 100% genetic similarity among the mother plant and their regenerants. While for the epigenetic changes in the samples, the MS-ISSR markers detected DNA methylation (2.7%) in the regenerants compared to the mother plant (1%), whereas MS-RAPD markers detected a little methylation patterns in the regeneratns (0.6%) compared to the mother plants (0.4%).
Bangladesh J. Bot. 44(1): 45-50, 2015 (March)
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© Bangladesh Botanical Society
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