Effect of plant growth regulators on plant regeneration of hyacinth bean (Lablab purpureus (L.) Sweet) through nodal explants

  • MT Islam Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur-1701, Bangladesh
  • MS Haque Department of Biotechnology, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh
  • S Rahman Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur-1701, Bangladesh
  • MR Molla Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur-1701, Bangladesh
  • ERJ Keller In Vitro Storage and Cryopreservation, Department of Genebank, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), OT Gatersleben, Correns Strasse 3, D-06466 Stadt Seeland, Germany.
Keywords: Lablab purpureus, nodal explant, growth regulators, regeneration

Abstract

Nodal explants of three accessions namely BD-101, BD-122 and BD-8001 of hyacinth bean (Lablab purpureus (L.) Sweet) were cultured for four weeks on MS medium supplemented with different concentrations and combinations of NAA (0, 0.1 mgl-1) and BAP (0.5, 1.0 and 2.5 mgl-1) for plant regeneration. The highest shoot initiation was observed in 0.5 mgl-1 BAP while the lowest shoot initiation was found in 2.5 mgl-1 BAP with 0.1 mgl-1 NAA. Earlier shoot initiation was exhibited in 0.5 mgl-1 BAP with 0.1 mgl-1 NAA. The highest number of leaflets and higher shoot lengths were observed in MS medium. Comparatively higher number of shoots was found in BAP (0.5-2.5 mgl-1). The highest percentage of callus was initiated in medium supplemented with 1.0 mgl-1 BAP. Earlier callus initiation and larger callus size were found in combination of BAP (0.5-2.5 mgl-1) with 0.1 mgl-1 NAA. BD-122 cultured in MS medium was found superior for shoot regeneration through node culture. Four different concentrations of IBA (0.1-1.5 mgl-1) were used for rooting. The highest percentage (86.67 %) of rooting was found in MS medium containing 0.1 mgl-1 IBA at four weeks. Rooting frequency decreased with the increasing concentration of IBA. The accession BD-8001 had 99.60 % rooting in 0.5 mgl-1 IBA. The highest number of longest roots was exhibited in 0.1 mgl-1 IBA. The regeneration protocol developed from nodal explants has applicability in improvement of hyacinth bean.

Bangladesh J. Agril. Res. 44(1): 27-42, March 2019

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Abstract
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Published
2019-04-07
How to Cite
Islam, M., Haque, M., Rahman, S., Molla, M., & Keller, E. (2019). Effect of plant growth regulators on plant regeneration of hyacinth bean (Lablab purpureus (L.) Sweet) through nodal explants. Bangladesh Journal of Agricultural Research, 44(1), 27-42. https://doi.org/10.3329/bjar.v44i1.40901
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Articles