Performance of different protocols on in vitro tuberization in potato (Solanum tuberosum)

Abstract

Five protocols of micro tuberization were used to induce large size microtuber in three recommended potato varieties, namely Cardinal, Diamant, and Heera under complete dark condition. Tuberization was the earliest (11.8 days) in the protocol P₂ (MS + 5 mg/l BAP + 500 mg/l CCC + 8% sucrose), which was closely followed by that in P₁ (12.7 days) (MS + 5 mg/l BAP + 50 mg/l coumarin + 8% sucrose). Maximum number of microtubers/flask (12.8) was obtained from the protocol P₁ followed by that of P₂ (11.6) that contained growth retardant; but higher average weight of microtuber was obtained in the protocols P₅ (30 days old plantlet + MS media containing 40 meq K + 10 mg/l BA + 9% sucrose), P₄ (MS + 10 mg/l BA + 8% sucrose), and P₃ (MS + 5.0 mg/l BAP + 6% sucrose) which contained BA in absence of growth retardant. The average weight of microtuber was the highest (329.0 mg) in protocol P₅, followed by that in P₄ (280.7 mg), while it was the lowest in protocol P1. The variety Diamant produced maximum average weight of microtuber (246.3 mg), while Heera produced minimum (226.1 mg), which was statistically similar to Cardinal (228.7 mg). The highest percentage (52.2) of >300 mg size and lowest percentage (19.3) of <150 mg size microtuber was produced in P₅ protocol in the variety Diamant. On overall consideration, all the varieties performed best with the protocol P₅.

DOI: http://dx.doi.org/10.3329/bjar.v39i1.20143

Bangladesh J. Agril. Res. 39(1): 59-66, March 2014