In vitro propagation of muskmelon (Cucumis melo L.) from nodal segments, shoot tips and cotyledonary nodes

  • S. Parvin University of Rajshahi
  • M. Kausar University of Rajshahi
  • M. Enamul Haque University of Rajshahi
  • M. Khalekuzzaman University of Rajshahi
  • B. Sikdar University of Rajshahi
  • M. Asadul Islam University of Rajshahi
Keywords: In vitro propagation, Nodal segments, Shoot's tip, Cotyledonary nodes, Cucumis melo.

Abstract

A rapid and efficient protocol is outlined for in vitro propagation of muskmelon
(Cucumis melo L.) Shoot tips, nodal segments and cotyledonary nodes from in
vitro grown seedlings were used as explants. The explants were inoculated on MS medium fortified with different combinations and concentrations of growth
regulators viz., BAP, NAA, GA3 and IBA for multiple shoot regeneration.
Effective result was found on MS medium supplemented with 2.0 mg/l BAP, in
which 90% and 70% cultures induced multiple shoots from nodal segments and
shoot tip explants, respectively. Whereas, 70% cultures of cotyledonary nodes
were found to induced shoots on MS medium with 1.5 mg/l BAP + 0.1 mg/l GA3. In vitro regenerated shoots were subcultured on half strength MS medium
supplemented with different concentrations of IBA and NAA for successful root
induction and the effective result (up to 70%) was found in medium with 1 mg/l
IBA. Well rooted in vitro grown plantlets were acclimatized in sandy soil, where
as 70% plantlets survived

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Author Biographies

S. Parvin, University of Rajshahi

Professor Joarder DNA & Chromosome Research Laboratory, Department of Genetic
Engineering and Biotechnology

M. Kausar, University of Rajshahi
Professor Joarder DNA & Chromosome Research Laboratory, Department of Genetic
Engineering and Biotechnology
M. Enamul Haque, University of Rajshahi
Professor Joarder DNA & Chromosome Research Laboratory, Department of Genetic
Engineering and Biotechnology
M. Khalekuzzaman, University of Rajshahi
Professor Joarder DNA & Chromosome Research Laboratory, Department of Genetic
Engineering and Biotechnology
B. Sikdar, University of Rajshahi
Professor Joarder DNA & Chromosome Research Laboratory, Department of Genetic
Engineering and Biotechnology
M. Asadul Islam, University of Rajshahi
Professor Joarder DNA & Chromosome Research Laboratory, Department of Genetic
Engineering and Biotechnology
Published
2015-01-15
How to Cite
Parvin, S., Kausar, M., Haque, M., Khalekuzzaman, M., Sikdar, B., & Islam, M. (2015). In vitro propagation of muskmelon (Cucumis melo L.) from nodal segments, shoot tips and cotyledonary nodes. Rajshahi University Journal of Life & Earth and Agricultural Sciences, 41, 71-77. https://doi.org/10.3329/rujleas.v41i0.21627
Section
Articles