TY - JOUR AU - Murthy, K. Sri Rama AU - Kondamudi, R. PY - 2012/01/24 Y2 - 2024/03/28 TI - Rapid Shoot Regeneration from Thin Cell Layer Explants of an Endangered Medicinal Asclepiad Ceropegia spiralis L. JF - Plant Tissue Culture and Biotechnology JA - Plant Tissue Cult. & Biotech. VL - 21 IS - 1 SE - Articles DO - 10.3329/ptcb.v21i1.9564 UR - https://www.banglajol.info/index.php/PTCB/article/view/9564 SP - 63-73 AB - <p>The thin cell layers of nodes and internodes of<em> Ceropegia spiralis</em> L. were cultured on&nbsp; MS&nbsp; supplemented with BAP 13.32 &micro;M/l + NAA 0.537 &mu;M/l induced 17.34 &plusmn; 0.55 shoots showing extensive growth. Later on the organogenesis was also induced on MS containing BAP 13.32 &micro;M/l + 2, 4-D 1.130 &micro;M/l, whereas the medium with BAP 13.32 &micro;M/l + 2, 4-D 4.52 &micro;M/l has the highest callus producing ability in recalcitrants as well as in normal explants. Shoots developed were rooted best on 0.5 MS with NAA 10.74 &mu;M/l. Optimum shoot and root multiplication was obtained within eight weeks.&nbsp; <em>In vitro</em> plantlets were successfully weaned and transferred to soil with about 90 per cent survival rate. So far, more than 650 weanlings have been produced successfully and reintroduced into nature for their recovery. &nbsp;</p> <p><em>&nbsp;</em></p> <p><em>Key words</em>: <em>Ceropegia spiralis,</em> Thin cell layer explants, Recalcitrants, Regeneration</p> <p><strong>&nbsp;</strong></p> <p>D. O. I. 10.3329/ptcb.v21i1.9564</p> <p><strong>&nbsp;</strong></p> <p>Plant Tissue Cult. &amp; Biotech. <strong>21</strong>(1): 63-73, 2011 (June)</p> ER -