TY - JOUR AU - Tan, Boon Chin AU - Chin, Chiew Foan AU - Alderson, Peter PY - 2012/01/24 Y2 - 2024/03/29 TI - An Improved Plant Regeneration of Vanilla planifolia Andrews JF - Plant Tissue Culture and Biotechnology JA - Plant Tissue Cult. & Biotech. VL - 21 IS - 1 SE - Articles DO - 10.3329/ptcb.v21i1.9560 UR - https://www.banglajol.info/index.php/PTCB/article/view/9560 SP - 27-33 AB - <p>Multiple shoots of <em>Vanilla planifolia</em> were induced from nodal explants under influence of different concentrations of plant growth regulators and coconut water (CW). A comparison of shoot regeneration between semi solid and liquid culture media was also investigated. &nbsp;Ninety seven per cent of the explants produced a mean number of 9.6 shoots with a mean shoot length of 4.70 cm when cultured on liquid MS supplemented with 1.0 mg/l BAP in combination of 15% CW. Shoots generated were rooted with frequency of 93% on MS supplemented with 1.0 mg/l NAA with a mean number of 2.9 roots per shoot and mean length of 4.0 cm within four weeks. The plantlets were transferred to sand : compost mixture (1 : 2) with 85.0% survival rate recorded after four weeks of acclimation.</p> <p><strong>&nbsp;</strong></p> <p><em>Key words:</em><strong> </strong>Regeneration, coconut water, nodal culture, vanilla</p> <p><strong>&nbsp;</strong></p> <p>D.O.I. 10.3329/ptcb.v21i1.9560</p> <p><strong>&nbsp;</strong></p> <p>Plant Tissue Cult. &amp; Biotech. <strong>21</strong>(1): 27-33, 2011 (June)</p> ER -