TY - JOUR AU - Akter, Nasrin AU - Sarkar, Selim AU - Hasan, Naimul AU - Rahman, Shaikh Mizanur AU - Rauf Sarkar, Md Abdur PY - 2022/12/29 Y2 - 2024/03/29 TI - Development of In vitro Mass Propagation Protocol for Gerbera (Gerbera jamesonii Bolus) var. Orange JF - Plant Tissue Culture and Biotechnology JA - Plant Tissue Cult. & Biotech. VL - 32 IS - 2 SE - Articles DO - 10.3329/ptcb.v32i2.63555 UR - https://www.banglajol.info/index.php/PTCB/article/view/63555 SP - 217-226 AB - <p>An <em>in vitro </em>mass propagation protocol was developed for Gerbera (<em>Gerbera jamesonii </em>Bolus) var. orange using flower bud explants. The young (7 days) explants were found to be suitable for culture and highest (100%) callus induction was obtained in MS medium supplemented with 5.0 mg/l BAP with 1.0 mg/l NAA. The maximum shoot regeneration (95%) and the number of shoots (10) were observed in MS medium supplemented with 6.0 mg/l BAP with 0.3 mg/l NAA. The highest shoot multiplication frequency (95%) and the number of shoots (60) were obtained in MS medium supplemented with only 2.0 mg/l BAP. The well-developed highest number of roots (5.0) per plant and average root length (5.0 cm) with 100% root induction efficiency without callusing was recorded in the half strength of MS medium devoid of any hormonal supplement. Acclimatized <em>in vitro </em>grown plantlets were successfully transferred into the field condition.</p><p>Plant Tissue Cult. &amp; Biotech. <strong>32</strong>(2): 217-226, 2022 (December)</p> ER -