TY - JOUR AU - Yesmin, Sabina AU - Hoque, MI AU - Sarker, RH PY - 2021/06/18 Y2 - 2024/03/29 TI - Enhanced Regeneration Through ex vitro Rooting and Agrobacterium-mediated Genetic Transformation of Eggplant (Solanum melongena L.) JF - Plant Tissue Culture and Biotechnology JA - Plant Tissue Cult. & Biotech. VL - 31 IS - 1 SE - Articles DO - 10.3329/ptcb.v31i1.54115 UR - https://www.banglajol.info/index.php/PTCB/article/view/54115 SP - 97-108 AB - <p>Regeneration of <em>in vitro </em>multiple shoots was achieved through organogenesis on MS supplemented with 2.0 mg/l BAP and 0.5 mg/l Kn from cotyledonary leaf explants of two local varieties of eggplant (<em>Solanum melongena </em>L.). Elongation of regenerated shoots was obtained on growth regulator free MS. <em>In vitro </em>root induction from excised regenerated shoots was less effective on MS with or without plant growth regulators. On the other hand regenerated shoots treated with 10 mM IBA for 5 min were found to be effective for <em>ex vitro </em>rooting in sterilized soil. Following sufficient development of roots, the <em>ex vitro </em>rooted plantlets were acclimatized in growth room condition, and were transferred to the field having 100% survival rate. The regeneration system developed was utilized for <em>Agrobacterium-</em>mediated genetic transformation using <em>Agrobacterium tumefaciens </em>strain LBA4404/pBI121 containing <em>GUS </em>and <em>nptII </em>genes. Adequate transformation response was obtained from cotyledonary leaf segments with bacterial suspension having an optical density of 0.50 at 600 nm with 30 min incubation followed by co-cultivation period of 72 hrs in Nayantara (BARI Begun-5) variety of eggplant. Selection of transformed shoots was carried out on MS supplemented with 2.0 mg/l BAP, 0.5 mg/l Kn, 300 mg/l carbenicillin and 100 mg/l kanamycin. Stable integration of <em>GUS </em>and <em>nptII </em>genes in Nayantara were confirmed through PCR analysis using the genomic DNA isolated from transformed shoots.</p><p>Plant Tissue Cult. &amp; Biotech. 31(1): 97-108, 2021 (June)</p> ER -