TY - JOUR AU - Knyazev, Aleksey AU - Kuluev, Bulat AU - Vershinina, Zilya AU - Chemeris, Aleksey PY - 2018/06/28 Y2 - 2024/03/28 TI - Callus Induction and Plant Regeneration from Leaf Segments of Unique Tropical Woody Plant Parasponia andersonii Planch JF - Plant Tissue Culture and Biotechnology JA - Plant Tissue Cult. & Biotech. VL - 28 IS - 1 SE - Articles DO - 10.3329/ptcb.v28i1.37197 UR - https://www.banglajol.info/index.php/PTCB/article/view/37197 SP - 45-55 AB - <p>The purpose of the present study was to develop effective methods for callus induction, shoot regeneration, and rooting for <em>Parasponia andersonii</em>. Leaf explants of <em>P. andersonii </em>were placed on Lloyd and McCown’s (WPM) medium supplemented with various concentrations of TDZ and NAA for callus induction. Callus induction was observed on media containing 0.1 - 0.2 mg/l TDZ with 0.05 mg/l NAA. Maximum shoot regeneration was observed when the calluses were cultured on MS supplemented with TDZ and IBA. Shoots cultured on WPM medium supplemented with 0.5 mg/l IBA had the maximum rooting percentage (100) in 3 weeks. Rooted plants were transplanted to a potting mixture containing vermiculite (50%) and peat (50%) (v/v). After 2 months, more than 20% of plants survived and were transferred to the greenhouse. Thus, a new effective method has been developed for <em>P. andersonii </em>micropropagation that can be used in studies of plant-<em>Rhizobium </em>symbiosis and for the generation of transgenic <em>Parasponia </em>plants.</p><p>Plant Tissue Cult. &amp; Biotech. 28(1): 45-55, 2018 (June)</p> ER -