TY - JOUR AU - Dwiyani, Rindang AU - Purwantoro, Aziz AU - Indrianto, Ari AU - Semiarti, Endang PY - 2015/07/09 Y2 - 2024/03/29 TI - Micropropagation of Orchid Carrying Knotted1-Like from Arabidopsis thaliana (Knat1) Gene JF - Plant Tissue Culture and Biotechnology JA - Plant Tissue Cult. & Biotech. VL - 25 IS - 1 SE - Articles DO - 10.3329/ptcb.v25i1.24121 UR - https://www.banglajol.info/index.php/PTCB/article/view/24121 SP - 13-20 AB - <p><em>KNOTED1-</em><em>LIKE from Arabidopsis thaliana </em>(<em>KNAT1</em>) gene keeps shoot apical meristem (SAM) in the meristematic state. The objective of the research was to investigate whether the function of <em>KNAT1 </em>gene in keeping plant cells under ‘meristematic state’ was functionally still work in the organ of the <em>KNAT1 </em>transformant. The transformant and WT plants were micropropagated, sliced and cut into some pieces, i.e. base of root, middle of root, root tip, a whole shoot, base of leaf, and leaf tip and planted in the New Phalaenopsis medium added with and without 5μM 2-iP and 0.15 μM NAA. The results show that cells of <em>KNAT1 </em>transformant were more meristematic compared to wild type plants as the organ produced more buds in micropropagation.</p><p>Plant Tissue Cult. &amp; Biotech. 25(1): 13-20, 2015 (June)</p> ER -