TY - JOUR AU - Nath, Sayantani AU - Das, Anusree PY - 2014/06/19 Y2 - 2024/03/29 TI - Direct Organogenesis and Genetic Characterization of Solanum pseudocapsicum L. in vitro Regenerated Plants JF - Plant Tissue Culture and Biotechnology JA - Plant Tissue Cult. & Biotech. VL - 24 IS - 1 SE - Articles DO - 10.3329/ptcb.v24i1.19201 UR - https://www.banglajol.info/index.php/PTCB/article/view/19201 SP - 65-76 AB - <p><em>Solanum pseudocapsicum</em> L. is an unexplored plant of Solanaceae having horticultural and medicinal importance. Earlier the <em>in vitro</em> morphogenic potential of this plant species has not been explored. Induction of direct organogenesis was obtained from internodes, leaf bases and root explants from <em>in vitro</em> grown seedlings and plants  in MS supplemented with different concentrations and combinations of BAP (0.44 - 8.88 µM/l) and IAA (0.29 - 2.85 µM/l). Direct organogenesis was achieved in 90% of internodes, 65% of leaf bases and 75% of root explants. The optimum regeneration medium was MS supplemented with 4.44 µM/l BAP and 1.43 µM/l IAA. The regeneration rate observed six weeks after culture was approximately 20, 9 and 12 shoot buds/explants from internodes, leaf bases and root explants, respectively. cDNA RAPD analysis from organogenic stages was carried out and revealed distinct gene expression pattern. <em>In vitro</em> rooting in 100% shoots was achieved on MS within ten days of culture. Successfully hardened rooted plants (R<sub>0</sub>) on transfer  to the field  produced flowers, fruits and viable seeds (R<sub>1</sub>). Genetic characterization of R<sub>0 </sub>and R<sub>1 </sub>plants revealed stability and uniformity at the chromosomal and genomic DNA level.</p> <p>Plant Tissue Cult. &amp; Biotech. <strong>24</strong>(1): 65-76, 2014 (June)</p> <p><strong> </strong></p> <p> </p><p>D. O. I. <a href="http://dx.doi.org/10.3329/ptcb.v24i1.19201">http://dx.doi.org/10.3329/ptcb.v24i1.19201</a></p> ER -