TY - JOUR AU - Hailu, Tilahun AU - Abera, Balcha AU - Mariam, Gabra PY - 2014/01/03 Y2 - 2024/03/28 TI - In vitro Mass Propagation of Artemisia (Artemisia annua L.) cv: Anamed JF - Plant Tissue Culture and Biotechnology JA - Plant Tissue Cult. & Biotech. VL - 23 IS - 2 SE - Articles DO - 10.3329/ptcb.v23i2.17518 UR - https://www.banglajol.info/index.php/PTCB/article/view/17518 SP - 165-176 AB - <p>An efficient <em>in vitro</em> propagation protocol was developed for anamed (A-3) cultivar of <em>Artemisia annua</em>. Two and 1.5% concentration of NaOCl treatment for 10 and 20 min were found to be optimum for sterilization of shoot tip and nodal explants, respectively. Maximum percentage (98.75 ± 2.50)<strong> </strong>shoot induction was observed from nodal explants cultured on MS supplemented with 0.8 mg/l BAP + 0.1 mg/l IBA  followed by 82.50 ± 2.88% from shoot tip explants on the same medium with 0.8 mg/l TDZ for shoot tip explants. The highest number of shoots (8.05 ± 0.66/explant) was regenerated on MS + 1 mg/l BAP + 0.1 mg/l IBA. Best rooting with mean values of 18.25 ± 0.95/explant root number and root length (6.35 ± 0.10 cm) was recorded on 1/2 MS + 0.5 mg/l IBA. Up on acclimation and transplanting, 80% survival efficiency was observed on the soil mix ratio of  2 : 1 : 1 (decomposed coffee husk, forest soil and sand, respectively). The developed regeneration protocol enables a large scale commercial production and a possible system towards the genetic improvement of this crop.</p> <p>D. O. I. <a href="http://dx.doi.org/10.3329/ptcb.v23i2.17518">http://dx.doi.org/10.3329/ptcb.v23i2.17518</a></p> <p>Plant Tissue Cult. &amp; Biotech. <strong>23</strong>(2): 165-176, 2013  (December)</p> ER -