TY - JOUR AU - Arumugam, A. AU - Gopinath, K. PY - 2013/07/03 Y2 - 2024/03/29 TI - In vitro Regeneration of an Endangered Medicinal Plant Withania somnifera using Four Different Explants JF - Plant Tissue Culture and Biotechnology JA - Plant Tissue Cult. & Biotech. VL - 23 IS - 1 SE - Articles DO - 10.3329/ptcb.v23i1.15564 UR - https://www.banglajol.info/index.php/PTCB/article/view/15564 SP - 79?85 AB - <div class="page" title="Page 1"><div class="layoutArea"><div class="column"><p><span>An efficient protocol was developed for highly regenerative capacity from leaf explant of </span><span>Withania somnifera </span><span>(L.) Dunal </span><span>? </span><span>an endangered medicinal plant. Calli were regenerated from four different explants like leaves, cotyledons, hypocotyls and epicotyls. MS supplemented with different concentrations of 2,4</span><span>?</span><span>D, BAP and NAA were used. The calli (94.33 ± 1.20%) were obtained from the leaf explant in 2,4</span><span>?</span><span>D 3.0 mg/l. The highest number of multiple shoots (85.67 ± 0.88%) were obtained from the leaf callus at 4.0 mg/l BAP. Shootlets forming calli were transferred to the rooting medium containing 10.0 mg/l NAA to produce multiple roots (89.33 ± 0.88%). The regenerated rooted shootlets were transferred to small polythene bags, which contain a sterilized cow</span><span>?</span><span>dung, sand and red soil (1 : 2 : 3) and kept in a mist house. After acclimation in the mist house the regenerated plantlets were hardened in the greenhouse and transferred to soil, which showed 85% survival rate. This new protocol was standardized for easy mass propagation of </span><span>W. somnifera </span><span>using leaf explant.</span></p><div class="page" title="Page 1"><div class="layoutArea"><div class="column"><div class="page" title="Page 1"><div class="layoutArea"><div class="column"><p><span>Plant Tissue Cult. &amp; Biotech. </span><span>23</span><span>(1): 79</span><span>?</span><span>85, 2013 (June)</span></p><p>DOI: <a href="http://dx.doi.org/10.3329/ptcb.v23i1.15564">http://dx.doi.org/10.3329/ptcb.v23i1.15564</a></p></div></div></div><p><span><br /></span></p></div></div></div></div></div></div> ER -