@article{Azad_Amin_2017, title={Regeneration of Asparagus officinalis L. Through Embryogenic Callus}, volume={27}, url={https://www.banglajol.info/index.php/PTCB/article/view/35008}, DOI={10.3329/ptcb.v27i1.35008}, abstractNote={<p>A plant regeneration system was established from hypocotyl explants of <em>in vitro </em>grown seedlings of <em>A. officinalis </em>and <em>in vitro </em>proliferated shoots, respectively through somatic embryogenesis and embryogenic calli. Somatic embryogenesis was significantly influenced by the types of plant growth regulators. Embryogenic calli with somatic embryos developed well in MS supplemented with 2.0 ‐ 4.0 μM BAP and 1.0 ‐ 4.0 μM 22,4‐D, NAA or IBA. The highest frequency (95.3%) of embryogenic calli and 55.2 somatic embryos formation were obtained when the MS was amended with 4.0 μM BAP and 2.0 μM 2,4‐D. The best embryo germination occurred in 1.0 μM BAP supplemented MMS. The highest 97.2% of shoot proliferation was observed in embryogenic calli in MS medium containing 2.0 μM BAP and 1.0 μM IBA. <em>In vitro </em>grown shoots were rooted in MMS with 1.0 ‐ 2.0 μM IBA. Regenerants were transferred to vermicompost and successfully established under an <em>ex vitro </em>environment in garden soil with 80% survival rate.</p><p>Plant Tissue Cult. & Biotech. 27(1): 21-31, 2017 (June)</p>}, number={1}, journal={Plant Tissue Culture and Biotechnology}, author={Azad, Mustafa Abul Kalam and Amin, Muhammad Nurul}, year={2017}, month={Dec.}, pages={21–31} }