Purification and Characterization of Urea Transporter by SDS-PAGE from the Paddy Grown in Urea Treated Soil

Authors

  • MS Haque Department of Biochemistry and Molecular Biology, Laboratory of Protein and Enzyme Research, University of Rajshahi, Rajshahi-6205, Bangladesh
  • MK Hasan Department of Biochemistry and Molecular Biology, Laboratory of Protein and Enzyme Research, University of Rajshahi, Rajshahi-6205, Bangladesh

DOI:

https://doi.org/10.3329/pa.v18i2.17463

Keywords:

Paddy extract, SDS-PAGE, Soil urea, Urea transporter

Abstract

G-50 chromatography of extract of paddy plant induced by urea revealed the major three peaks F-1, F-2 and F-3 against the absorbance at 280 nm with the number of fractions. Chromatogram was also obtained from the control of extract of paddy, however, urea-induced paddy extract showed the higher absorbance than the control showing the higher protein content. F-1 fraction for both groups having the higher molecules are considered to have the urea transporter and are applied to SDS-PAGE for purification and characterization. For identification of urea transporter, a mixture of marker proteins- ?-galactosidase, BSA, egg white albumin, pepsin, trypsin inhibitor and lysozyme were used. Depending on the mobility of the sample, urea-transporter had been identified as a large band having the MW of 50.6 kDa compared to the MW of BSA. For control, SDS-PAGE shows the same band for urea-transporter but the expression was lower than the urea-treated paddy. In SDS-PAGE gel, both bands were homogenous showing the homogeneity of urea-transporter. The results demonstrate that urea application in the soil induces the expression of urea transporter which will be a new finding for the clarification of mechanism of urea transport in plants.

DOI: http://dx.doi.org/10.3329/pa.v18i2.17463

Progress. Agric. 18(2): 41 - 47, 2007

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Published

2013-12-27

How to Cite

Haque, M., & Hasan, M. (2013). Purification and Characterization of Urea Transporter by SDS-PAGE from the Paddy Grown in Urea Treated Soil. Progressive Agriculture, 18(2), 41–47. https://doi.org/10.3329/pa.v18i2.17463

Issue

Section

Crop Science