Studies on <i>In vitro</i> Callus Induction and Regeneration from Nodal Explants of <i>Cassia alata</i> L.

Authors

  • MF Hasan Department of Genetic Engineering and Biotechnology, University of Rajshahi Rajshahi-6205, Bangladesh
  • MS Rahman Department of Genetic Engineering and Biotechnology, University of Rajshahi Rajshahi-6205, Bangladesh
  • MS Hossain Department of Genetic Engineering and Biotechnology, University of Rajshahi Rajshahi-6205, Bangladesh
  • M Rahman Department of Genetic Engineering and Biotechnology, University of Rajshahi Rajshahi-6205, Bangladesh

DOI:

https://doi.org/10.3329/pa.v19i1.17105

Keywords:

Callus induction, Shoot regeneration, Nodal explants, Cassia alata

Abstract

A successful protocol for adventitious shoot regeneration was developed from the nodal explant derived callus tissue of Cassia alata. Greenish friable callus was induced from the cut surface of the nodal explants on MS medium supplemented with 1.5 mgl-1 2,4-D within twenty days of inoculation. The calli differentiated into adventitious shoots when they were cultured on MS medium supplemented with 1.5 mgl-1 2,4-D and 0.5 mgl-1 Kn. In this treatment, the highest number of shoot induction per callus was 6.0 ±1.0. The callus derived shoots rooted on MS medium containing 1.0 mgl-1 IBA within ten days of culture. The in vitro grown plantlets were acclimatized and successfully transferred to natural condition with 80% survival.

DOI: http://dx.doi.org/10.3329/pa.v19i1.17105

Progress. Agric. 19(1): 39 - 44, 2008

 

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Published

2013-11-23

How to Cite

Hasan, M., Rahman, M., Hossain, M., & Rahman, M. (2013). Studies on <i>In vitro</i> Callus Induction and Regeneration from Nodal Explants of <i>Cassia alata</i> L. Progressive Agriculture, 19(1), 39–44. https://doi.org/10.3329/pa.v19i1.17105

Issue

Vol. 19 No. 1 (2008)

Section

Crop Science