Screening of protease producing bacteria from tannery wastes of leather processing industries at Hazaribag, Bangladesh

  • Md Saimoon Rahman Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208
  • Md Rayhan Islam Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208
  • Omit Kumer Mondol Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342
  • Mohammad Shahedur Rahman Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342
  • Farah Sabrin Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902
  • Umme Salma Zohora Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342
Keywords: Protease, 16s rRNA sequence, tannery w

Abstract

Protease enzymes are ubiquitous in nature and usually found in all living organisms. The present study was conducted for screening bacteria with the ability to produce extracellular proteases. Attempts were made to isolate protease producing bacterial strains from soil samples of tannery wastes from Hazaribag, Bangladesh and subsequent partial characterization was performed. A number of biochemical assays was performed for presumptive identification of the bacteria. Subsequent molecular identification of bacterial isolates was performed using 16S rRNA sequence analysis, which revealed that the isolated bacterium R82 to be Bacillus subtilis. The protease enzyme activity was determined 60.50 U/mL. Further characterization showed that the enzyme produced from the bacteria was extracellular and alkaline protease in nature. The optimum reaction incubation time, pH and temperature were found to be 30 min, 8.0 and 50°C, respectively. Bacterial culture supernatant was used to carry out dehairing of leather (2ʹʹ×2ʹʹ), which was achieved in 24 h. The results showed that isolated bacterial strain could be employed for strain improvement towards commercial production of protease with a promise for dehairing in leather processing industries.

Jahangirnagar University J. Biol. Sci. 7(1): 23-34, 2018 (June)

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Abstract
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PDF
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Published
2018-08-18
How to Cite
Rahman, M. S., Islam, M. R., Mondol, O., Rahman, M. S., Sabrin, F., & Zohora, U. (2018). Screening of protease producing bacteria from tannery wastes of leather processing industries at Hazaribag, Bangladesh. Jahangirnagar University Journal of Biological Sciences, 7(1), 23-34. https://doi.org/10.3329/jujbs.v7i1.37970
Section
Original Research Articles