Repeated batch fermentation for protease production using biofilm fermentation of Bacillus sp.

  • Ripa Moni Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342
  • Mohammed Salahuddin Life Science Division, Overseas Marketing Corporation (Pvt.) Limited, Dhaka
  • Md Abdullah Al Noman Khan Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342
  • Umme Salma Zohora Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342
  • Mohammad Shahedur Rahman Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342
Keywords: Bacillus subtilis, biofilm, repeated batch, protease enzyme

Abstract

Repeated batch fermentation is a stable, economically viable and environment friendly fermentation system. Biofilm of Bacillus subtilis was investigated for repeated batch fermentation for the production of protease enzyme. It was found that complete replacement of spent medium with fresh medium supplementation in biofilm produced similar amount of protease that is about 225 U/ml of enzyme in successive batches. Moreover, unlike some solid carriers the EPS layer does not entrap much enzyme inside it. Therefore, instead of using immobilized cells, biofilm of B. subtilis can be a good candidate for protease enzyme production using repeated batch fermentation.

Jahangirnagar University J. Biol. Sci. 6(2): 29-38, 2017 (December)

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Abstract
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Published
2018-05-07
How to Cite
Moni, R., Salahuddin, M., Khan, M. A., Zohora, U., & Rahman, M. S. (2018). Repeated batch fermentation for protease production using biofilm fermentation of Bacillus sp. Jahangirnagar University Journal of Biological Sciences, 6(2), 29-38. https://doi.org/10.3329/jujbs.v6i2.36588
Section
Original Research Articles