Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk.

Authors

  • S Yesmin National Institute of Biotechnology, Ganakbari, Savar, Dhaka-1349
  • A Hashem National Institute of Biotechnology, Ganakbari, Savar, Dhaka-1349
  • MS Islam Bangladesh Atomic Energy Commission, E-12/A, Sher-e-Bangla Nagar, Dhaka-1207

DOI:

https://doi.org/10.3329/jujbs.v4i1.27786

Keywords:

Eclipta alba, nodal explant, Micropropagation, In vitro, acclimatization

Abstract

Nodal segments from naturally grown Eclipta alba (L.) Hassk.were used as explants for organogenesis. Multiple shoots were obtained from the explants cultured on MS medium supplemented with various concentrations of BAP and Kn alone or in combination with NAA and IAA. Maximum number of multiple shoots (18.40±0.67) were induced on MS medium supplemented with 1.0 mg/l BAP and 0.1mg/NAA. In vitro raised shoots were cultured onto half and full strength MS medium supplemented with different concentration of IBA, IAA and NAA. The best root induction medium was found to be half strength MS containing 0.1 mg/l IBA where 96% shoots rooted. Regenerated plantlets grew normally without showing any morphological variation and flowered after 45 days of transplantation.

Jahangirnagar University J. Biol. Sci. 4(1): 61-69, 2015 (June)

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Author Biography

S Yesmin, National Institute of Biotechnology, Ganakbari, Savar, Dhaka-1349



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Published

2016-05-18

How to Cite

Yesmin, S., Hashem, A., & Islam, M. (2016). Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk. Jahangirnagar University Journal of Biological Sciences, 4(1), 61–69. https://doi.org/10.3329/jujbs.v4i1.27786

Issue

Vol. 4 No. 1 (2015)

Section

Original Research Articles

License

(c) Jahangirnagar University Journal of Biological Sciences.  

Articles in the Jahangirnagar University Journal of Biological Sciences are Open Access articles published under the Creative Commons CC BY License (https://creativecommons.org/licenses/by/4.0/). This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.