Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk.
Nodal segments from naturally grown Eclipta alba (L.) Hassk.were used as explants for organogenesis. Multiple shoots were obtained from the explants cultured on MS medium supplemented with various concentrations of BAP and Kn alone or in combination with NAA and IAA. Maximum number of multiple shoots (18.40±0.67) were induced on MS medium supplemented with 1.0 mg/l BAP and 0.1mg/NAA. In vitro raised shoots were cultured onto half and full strength MS medium supplemented with different concentration of IBA, IAA and NAA. The best root induction medium was found to be half strength MS containing 0.1 mg/l IBA where 96% shoots rooted. Regenerated plantlets grew normally without showing any morphological variation and flowered after 45 days of transplantation.
Jahangirnagar University J. Biol. Sci. 4(1): 61-69, 2015 (June)
Copyright (c) 2016 Jahangirnagar University Journal of Biological Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
(c) Jahangirnagar University Journal of Biological Sciences.
Articles in the Jahangirnagar University Journal of Biological Sciences are Open Access articles published under the Creative Commons CC BY License (https://creativecommons.org/licenses/by/4.0/). This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.