A Comparative Study of LAMP and PCR in Relation to Time and Cost

Authors

  • Tanzila Rawnuck Assistant Professor, Dept of Pathology with Microbiology, Dhaka Dental College, Dhaka, Bangladesh
  • Md Selim Reza Assistant Professor, National Institute of Traumatology & Orthopaedic Rehabilitation (NITOR), Dhaka, Bangladesh.
  • Mohammad Jahidur Rahman Khan Assistant Professor, Dept of Microbiology, Shaheed Suhrwardy Medical College, Dhaka, Bangladesh
  • Rashida Akter Khanam Associate Proffesor, Dept of Microbiology, Shaheed Suhrwardy Medical College, Dhaka, Bangladesh
  • Saif Ullah Munshi Professor & Head of the Department, Dertment of Virology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh

DOI:

https://doi.org/10.3329/jssmc.v12i2.56885

Keywords:

Loop-mediated isothermal amplification, qPCR, Low cost, Short-time operation.

Abstract

Background: The Loop-mediated isothermal amplification (LAMP) represents a very sensitive, easy to use, and less time consuming diagnostic method.

Aims: The aim was to establish a simple, cost-effective, molecular technique.

Materials and methods: An analytical study was conducted using two hundred acute serum samples using two different molecular techniques; qPCR and LAMP to standardize a costeffective and less time-consuming technique.

Results: The cost of in-house LAMP reagents was one-ninth of the cost of commercial qPCR. Consume cost was 23 times less than qPCR besides, lab setup cost was 92 times less than qPCR. More importantly, LAMP requires 5-6 times less time duration than qPCR.

Conclusion: Due to its simple short-time operation with low cost, it would be a prevalent molecular technique globally, particularly in Bangladesh.

J Shaheed Suhrawardy Med Coll 2020; 12(2): 72-75

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Published

2022-01-03

How to Cite

Rawnuck, T. ., Reza, M. S. ., Rahman Khan, M. J. ., Khanam, R. A., & Munshi, S. U. . (2022). A Comparative Study of LAMP and PCR in Relation to Time and Cost. Journal of Shaheed Suhrawardy Medical College, 12(2), 72–75. https://doi.org/10.3329/jssmc.v12i2.56885

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Section

Original Articles