TY - JOUR AU - Marka, R. AU - Malothu, G. S. AU - Nanna, R. S. PY - 2022/05/01 Y2 - 2024/03/29 TI - Transgenic Peanut (Arachis hypogaea L.) Plants Conferring Enhanced Protection Against Fungal Pathogens by Expressing Tc chitinase-I Gene JF - Journal of Scientific Research JA - J. Sci. Res. VL - 14 IS - 2 SE - Section B: Chemical and Biological Sciences DO - 10.3329/jsr.v14i2.56542 UR - https://www.banglajol.info/index.php/JSR/article/view/56542 SP - 625-640 AB - <p>In this study, an efficient <em>Agrobacterium-</em>mediated genetic transformation method followed by <em>Tc chitiI</em> gene for enhanced expression in peanut (<em>Arachis hypogaea</em> L.) cv ICG 7827 is reported. <em>A. tumefaciens</em> strain LBA 4404 harboring the plant transformation plasmid pBinAR contains the <em>Tc chitI </em>gene and <em>nptII</em> gene as a selectable marker. The precultured leaflet (LL) explants were infected with <em>A. tumefaciens</em> containing <em>Tc chitiI</em> gene and cocultivated on SIM (MMS+10 mg/L BAP+1 mg/L NAA+2 mg/L AgNO<sub>3</sub>) for four days. After co-cultivation, these explants were transferred to selection medium (SIM+75 mg/L Kan+250 mg/L Cefotaxime). In T<sub>0</sub> generation, 70 % of transformation efficiency was recorded. T<sub>1</sub> generation derived from the primary transgenic (T<sub>0</sub>) events revealed a Mendelian inheritance pattern (3:1) for the <em>Tc chit I</em> transgene. T<sub>1</sub> transgenic peanut plants were tested for resistance against <em>C. arachidicola</em>,<em> C. personatum, </em>and <em>P. arachidis </em>by infection with the microspores using detached leaf assay. The <em>Tc chitI</em> gene expressed in T<sub>1 </sub>transgenic plants have shown longer incubation, longer latent period, and lower infection frequencies than non-transformed (WT) plants. A significant negative correlation was recorded between chitinase activity and the frequency of infection to the <em>C. arachidicola</em>,<em> C. personatum</em> and <em>P. arachidis</em> pathogens.</p> ER -