Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma <p>The Dhaka University Journal of Pharmaceutical Sciences is published by the Faculty of Pharmacy, University of Dhaka, Bangladesh. Full text articles available</p> <p><strong>Journal Metrics</strong><br><a href="https://www.scopus.com/sources?sortField=metric&amp;metricName=&amp;sortDirection=ASC&amp;offset=&amp;displayAll=true&amp;sortPerformedState=f&amp;origin=sourceSearch&amp;sortDirectionMOne=&amp;sortDirectionMTwo=&amp;sortDirectionMThree=&amp;metricDisplayIndex=1&amp;scint=1&amp;menu=search&amp;tablin=&amp;searchWithinResultsDefault=t&amp;searchString=&amp;searchOA=&amp;typeFilter=d_j_p_k&amp;subscriptionFilter=s_u&amp;filterActTriggered=f&amp;tabName=searchSources&amp;searchTermsSubmit=&amp;searchType=issn&amp;searchTerms=1816-1820" target="_blank" rel="noopener">CiteScore Tracker 2017</a>: Updated on January 28, 2019&nbsp; <img src="/public/site/images/admin/index11.jpg"></p> <p><a href="https://www.researchgate.net/journal/1816-1839_Dhaka_University_Journal_of_Pharmaceutical_Science" target="_blank" rel="noopener">ResearchGate Journal Impact</a>&nbsp;(2015): 0.52 <img src="/public/site/images/admin/Research_Gate1.jpg"></p> <p><a href="https://www.scimagojr.com/journalsearch.php?q=19700174893&amp;tip=sid" target="_blank" rel="noopener">Scimago Journal Rank</a> (2017): 0.18 <img src="/public/site/images/admin/j.jpg" width="155" height="156"></p> <p><a href="http://miar.ub.edu/issn/1816-1820" target="_blank" rel="noopener">MIAR Analysis</a> : Updated on January 28, 2019 <img src="/public/site/images/admin/MIAR.jpg"></p> Dhaka University en-US Dhaka University Journal of Pharmaceutical Sciences 1816-1820 <p>© Dhaka University Journal of Pharmaceutical Sciences</p> <p><a href="http://creativecommons.org/licenses/by-nc-nd/4.0/" rel="license"><img style="border-width: 0;" src="https://i.creativecommons.org/l/by-nc-nd/4.0/88x31.png" alt="Creative Commons Licence"></a><br>Articles in DUJPS are licensed under a <a href="http://creativecommons.org/licenses/by-nc-nd/4.0/" rel="license">Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License</a>.</p> Antioxidant Activities, Phenolic and Flavonoid Contents of Methanolic Extract of Stelechocarpus burahol Fruit and its Fractions https://www.banglajol.info/index.php/JPharma/article/view/39170 <p>Kepel or <em>Stelechocarpus burahol </em>is an exotic fruit originating from Java, Indonesia. This research was intended to evaluate the antioxidant activities of extracts and fractions of kepel fruit pulp (KFP) based on radical scavenging capability towards 2,2′-azinobis(3-ethylbenzo thiazoline-6-sulphonic acid) diammonium salt (ABTS) and 2,2′-diphenildiphenyl-1-picrylhydrazil radical (DPPH). In this study, the radical scavenging activities were also correlated with total phenolics and flavonoid contents. Among the evaluated samples, the ethyl acetate fraction exhibited the highest antiradical antioxidant activity towards ABTS radical with IC<sub>50</sub> of 0.35 μg/ml, lower than that of vitamin C (as positive control). It also showed highest antiradical activity using DPPH based assay. Methanolic extract had total phenolic content of 58.28 ± 0.37% wt/wt gallic acid equivalent, higher than its fraction. Meanwhile the petroleum ether soluble fraction revealed flavonoid content (76.06 ± 11.9%) as rutin equivalent, among the extract and fractions evaluated. Based on coefficient (R2) values, phenolics and flavonoids contents contributed to 73.09% and 30.99%% towards antiradical scavenging activities, respectively.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 153-159, 2018 (December)</p> Nur Herlina Sugeng Riyanto Sudibyo Martono Abdul Rohman ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 153 159 10.3329/dujps.v17i2.39170 In vitro Test of Macrophage Phagocytic Activity of Extracts and Fractions of Red Dragon Fruit Peel [Hylocereus polyrhizus (F.A.C.Weber) Britton and Rose] https://www.banglajol.info/index.php/JPharma/article/view/39171 <p>The peel of red dragon fruit [<em>Hylocereus polyrhizus </em>(F.A.C.Weber) Britton and Rose] can be used to treat various diseases and to improve immune system of body. This study was aimed to investigate the <em>in vitro </em>macrophage phagocytic activity of extracts and fractions of red dragon fruit peel (<em>Hylocereus polyrhizus</em>). The <em>in vitro </em>test was conducted based on the method of Leijh <em>et al. </em>The parameters of phagocytic activity were based on the macrophages capacity to phagocytose latex beads using the calculation of phagocytic capacity and phagocytic index. The test results indicated significant difference (p &lt; 0.05). The petroleum benzene extract showed higher phagocytic activity of macrophages than methanol extract of the fruit peel, sediment, and media control (-). The LSD test showed that macrophage phagocytic activity using fractions (500 and 100 μg/ml) was significantly different from macrophage phagocytic activity using fractions (20 μg/ml), sediment (500, 100 and 20 μg/ml), extracts (500, 100 and 20 μg/ml), and media control.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 161-165, 2018 (December)</p> Sri Wahdaningsih Subagus Wahyuono Sugeng Riyanto Retno Murwanti ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 161 165 10.3329/dujps.v17i2.39171 Studies of Degradation Kinetics of a 5-HT3 Antagonist, Ramosetron Hydrochloride: Effects of Temperature https://www.banglajol.info/index.php/JPharma/article/view/39172 <p>Ramosetron hydrochloride is the hydrochloride salt of ramosetron, a selective serotonin (5-HT3) receptor antagonist with potential antiemetic activity. Upon administration, ramosetron selectively binds to and blocks the activity of 5-HT subtype 3 (5-HT<sub>3</sub>) receptors located in the vagus nerve terminal and the vomiting center of central nervous system (CNS), suppressing chemotherapy-induced nausea and vomiting. Degradation of Ramosetron HCl was conducted with 0.1N NaOH at 60<sup>°</sup>C, 70<sup>°</sup>C and 80<sup>°</sup>C to study the reaction kinetics. The reaction rate constants (k) for degradation at 60<sup>°</sup>C, 70<sup>°</sup>C and 80<sup>°</sup>C were -2.2680 molL<sup>-1</sup>s<sup>-1</sup> , -3.3714 molL<sup>-1</sup>s<sup>-1</sup> and -5.3686 molL<sup>-1</sup>s<sup>-1</sup> for zero order and -1.05 x 10<sup>-2</sup>s<sup>-1</sup>, -1.60 x 10<sup>-2</sup>s<sup>-1</sup> and -2.70 x 10<sup>-2</sup>s<sup>-1</sup> for first order kinetics, respectively. The activation energy of Ramosetron HCl was found as 10.05 kcalmol<sup>-1</sup> by using Arrhenius equation.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 167-173, 2018 (December)</p> Md Mokaram Hossain Reza ul Jalil Mohammad A Rashid ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 167 173 10.3329/dujps.v17i2.39172 Development and Validation of a RP-HPLC Method for Quantitative Analysis of Linagliptin in Bulk and Dosage Forms https://www.banglajol.info/index.php/JPharma/article/view/39173 <p>This research was aimed to establish a versatile, sensitive, rapid and validated RP-HPLC method to analyze linagliptin in bulk as well as in pharmaceutical dosage forms. Liquid chromatography was performed on HPLC system and 20μl of samples were injected into a C<sub>18</sub> column (150 x 4.6 mm i.d., 5μm particle size) and the eluents were monitored through a PDA detector at 239 nm. An isocratic method with a flow rate of 1 ml/min was used to elute the compounds with a mobile phase comprised of 70:30 v/v mixture of phosphate buffer (pH 6.8±0.2) and acetonitrile. The retention time of the compound was found to be 2.8 minutes. According to the ICH Q2(R1) guidelines, the method was validated by establishing several analytical parameters such as system suitability, specificity, linearity, accuracy, precision, limit of detection (LOD), limit of quantitation (LOQ), ruggedness and robustness to assay linagliptin. The method showed good linearity (R<sub>2</sub> = 0.9981) over the concentration ranges of 40 – 60 μg/ml with a recovery between 99.48% ± 0.38% RSD to 100.22% ± 0.011% RSD, whereas the LOD and LOQ values were 0.05 μg/ml and 0.15 μg/ml, respectively. The relative standard deviation (% RSD) for inter-day and intra-day precision was not more than 2.0%. Hence, the proposed method can be applied accurately for research and routine analysis of linagliptin in bulk as well as different pharmaceutical dosage forms.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 175-182, 2018 (December)</p> Joy Chandra Rajbangshi Md Mahbubul Alam Md Shahadat Hossain Md Samiul Islam Abu Shara Shamsur Rouf ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 175 182 10.3329/dujps.v17i2.39173 Impact Assessment of Different Polymers on Physicochemical Properties of Ibuprofen Loaded Solid Dispersions https://www.banglajol.info/index.php/JPharma/article/view/39174 <p>In the present study, solid dispersions of ibuprofen were prepared to improve aqueous solubility of ibuprofen. A series of formulations were prepared where PEG 6000 with polymers named PVP K30, cross PVP, poloxamer 237, HPMC ASLF, pregelatinized starch, Na-CMC, Eudragit L100, and kollidon IR were used in different ratios. Among 41 formulations, solid dispersions of ibuprofen in PEG 6000 with each of PVP K30, poloxamer 237, and Na-CMC at ratio of 2:9:7 revealed improved solubility of 952.73 ± 1.31, 878.18 ± 0.97, and 1263.64 ± 1.58 μg/ml, respectively. The physicochemical properties of these preparations were ascertained by FTIR, SEM, DSC, and particle size analyses. FTIR spectrum showed absence of chemical interactions and physical compatibilities between ibuprofen and polymers were confirmed by DSC. Disappearance of individual surface properties in solid dispersions were revealed by SEM studies, which indicated the formation of effective preparations. On the other hand, particle size analysis showed reduction in particle size of ibuprofen from solid dispersions that demonstrated solubility enhancement of ibuprofen. The above studies suggested that solid dispersions of ibuprofen in PEG 6000 at ratios of 2:9:7 with each of PVP K30, poloxamer 237, and Na-CMC were found to be effective to improve aqueous solubility.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 183-190, 2018 (December)</p> Sonia Ferdousy BK Sajeeb Shahida Yeasmin ABM Faroque ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 183 190 10.3329/dujps.v17i2.39174 Clerodendrum inerme (L.) Gaertn. Extract Exerts Anticancer Activity on Lung Cancer Cells https://www.banglajol.info/index.php/JPharma/article/view/39175 <p>Cancer is the leading cause of death word wide. Recently there are no new drugs for safe and efficient treatment. <em>Clerodendrum inerme </em>(L.) Gaertn. (Verbenaceae) plant is being used by the ethnic people for cancer treatment. In this study, cytotoxic and antiproliferative potential of hydroalcoholic (methanol and water; 70:30 v/v) extract of <em>C. inerme </em>were evaluated. Various anticancer investigations performed like, lung cancer cell A-549 culture, dye exclusion assay, MTT assay, morphological changes and compatibility with RBC, confirmed the presence of the moiety that have the cytotoxic and antiproliferative potential. Compatibility with RBC was observed, when treated with standard drug doxorubicin, and hydroalcoholic extract of <em>C. inerme </em>at 259.5 μg/ml concentration (IC<sub>50</sub>). In addition, the same treatment reveled, decrease in cytotoxic efficacy and cell viability against lung cancer cells. Furthermore, change in the cell morphology also suggesting potent antitumor properties of <em>C. inerme</em>.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 191-196, 2018 (December)</p> Mahendra Kumar Chouhan Pramod Jayadevappa Hurakadle Harsha Vasudev Hegde ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 191 196 10.3329/dujps.v17i2.39175 In vitro Activity of Rambutan Binjai (Nephelium lappaceum) Peel Extract from Indonesia to Methicillin-Resistant Staphylococcus aureus (MRSA) https://www.banglajol.info/index.php/JPharma/article/view/39176 <p>Methicillin-resistant <em>Staphylococcus aureus </em>(MRSA) is the most common bacteria causing nosocomial infections with high levels of resistance to available antibiotics. So, it is necessary to search for new compounds to solve this problem. Various studies showed antibacterial activity of rambutan peel but for Rambutan Binjai peel extract that are from Indonesia has never been studied against the MRSA. This study aims to determine the antibacterial activity, the value of minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) using agar diffusion method. The concentration of rambutan peel ethanol extract at as much as 62.5 mg/ml showed the inhibitory diameter i.e 21.3 ± 2.4 mm. MIC and MBC were in the same range, which was between 0.98 (mg/ml) to 1.95 (mg/ml). The activity strength of tetracycline against the extract was at 1:50. This revealed that Rambutan Binjai peel extract had great potency as antibacterial agent to MRSA.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 197-203, 2018 (December)</p> Tina Rostinawati Ami Tjitraresmi Myra Vania Wisnuputri ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 197 203 10.3329/dujps.v17i2.39176 Comparative Antioxidative and Antihyperglycemic Profiles of Pneumatophores of Two Mangrove Species Avicennia alba and Sonneratia apetala https://www.banglajol.info/index.php/JPharma/article/view/39177 <p>Ethanolic extracts of pneumatophores of two mangrove species- <em>Avicennia alba </em>and <em>Sonneratia apetala </em>were studied <em>in vitro </em>for antioxidant capacity by measuring their ability to scavenge free radicals and determining total phenolic, flavonoid and tannin contents. <em>In vivo </em>measurement of antihyperglycemic activity of extracts was done by oral glucose tolerance test. In considering the antioxidant activity, <em>S. apetala </em>extract showed superior IC50 (concentration of sample required to inhibit 50% of free radicals) value for scavenging DPPH radical (71.77 μg/ml), hydrogen peroxide radical (97.27 mg/l), hydroxyl radical (79.62 mg/l) and superoxide anion (108.89 mg/l). For <em>A. alba</em>, the values for the radical scavenging assays were much higher. In addition, total phenol, flavonoid and tannin content demonstrated by <em>S. apetala </em>were 204.03 mgGAE/g, 228.68 mgQE/g and 235.89 mgGAE/g whereas for <em>A. alba</em>, they were 65.52 mgGAE/g, 44 mgQE/g and 37.71 mgGAE/g, respectively. In oral glucose tolerance test, <em>S. apetala </em>reduced the blood glucose level to a higher extent than <em>A. alba</em>. So, <em>S. apetala </em>with higher amount of secondary metabolites (phenol, flavonoid, tannin) is a superior source of natural antioxidants and antihyperglycemics.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 205-211, 2018 (December)</p> Biswajit Biswas Mimi Golder Tannami Islam Samir Kumar Sadhu ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 205 211 10.3329/dujps.v17i2.39177 Polyherbal Antioxidant Topical Preparation Comprising Ethanol Extract of Tetracarpidium conophorum and Ocimum gratissimum: Formulation and Evaluation https://www.banglajol.info/index.php/JPharma/article/view/39178 <p>The use of antioxidants is an effective approach to prevent symptoms related to photo-induced aging of the skin. The aim of this research work was to formulate and evaluate a polyherbal antioxidant face cream using the ethanol extracts of <em>Tetracarpidium conophorum </em>and <em>Ocimum gratissimum</em>. The ethanol extract of the herbs was incorporated at varying concentrations into six different emulsion bases. Antioxidant activity of the formulations was assessed using 2,2-diphenyl-1-picrylhydrazyl method. The formulations were evaluated for pH, viscosity, spreadability and microbial content. Accelerated stability tests were performed on all the formulations to assess stability at varying storage conditions. All the formulations showed good spreadability, good consistency, homogeneity, appearance, pH and no phase separation occurred. Non-Newtonian pseudo-plastic flow influenced by increased shear was experienced by all the formulations. Concentration dependent antioxidant activity was observed with FC2 and FC4 showing the highest antioxidant activity with IC<sub>50</sub> value of 80.1 and 83.2 μg/ml, respectively. The polyherbal antioxidant preparation containing extracts of <em>T. conophorum </em>and <em>O. gratissimum </em>shown to exhibit excellent antioxidant properties. It can serve to protect the skin from reactive oxygen species created by UV radiation and environmental toxin, thus protecting the skin from photo aging.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 213-219, 2018 (December)</p> Margaret O. Ilomuanya Thomas Akhimien Chinelo Aghaizu Oluwatobiloba Adeyinka Tolulope Ajayi ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 213 219 10.3329/dujps.v17i2.39178 Synthesis, Antibacterial and Antifungal Evlaution of Novel Pyrazoline Derivatives https://www.banglajol.info/index.php/JPharma/article/view/39179 <p>A new series of chalcones (<strong>2a-j</strong>) were prepared by reacting substituted aldehydes and substituted ketones in alcohol medium in presence of NaOH. The chalcones underwent selective cyclization with guanicol hydrazide (<strong>1</strong>) in glacial acetic acid medium to yield the title compounds 1,3,5-trisubstituted pyrazolines (<strong>3a-j</strong>). The new compounds were characterized on the basis of 1H-NMR, IR and mass spectral data. All the newly synthesized compounds were evaluated for their <em>in-vitro </em>antibacterial and antifungal activities. Some of the tested compounds <strong>3a </strong>and <strong>3e </strong>showed good activity against bacterial strains and compounds <strong>3d </strong>and <strong>3h </strong>revealed good activity against fungal strains.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 221-226, 2018 (December)</p> BC Revanasiddappa MS Jisha M Vijay Kumar Hemanth Kumar ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 221 226 10.3329/dujps.v17i2.39179 Comparative Nephroprotective Effects of Extracts of Buchholzia coriacea on some Biochemical Parameters in Carbon Tetrachloride-induced Toxicity in Rattus novergicus https://www.banglajol.info/index.php/JPharma/article/view/39180 <p><em>Buchholzia coriacea </em>(wonderful kola) is a medicinal plant that has been used worldwide as an alternative medication to promote human health. Comparative nephroprotective effects of crude seed powder, aqueous and methanolic extracts of <em>B. coriacea </em>in hepatotoxic rats was investigated for 56 days. The crude powder (BCCP), aqueous extract (BCAE) and methanolic extract (BCME) significantly reduced (p&lt;0.05) the levels of creatinine and uric acid and decreased minimally throughout the treatment periods. Total protein increased significantly (p&lt;0.05). Significant decrease (p&lt;0.05) were observed with 200 mg/kg BCAE and BCCP respectively in urea and BUN levels. Noticeable nephroprotective effects may be attributed to the presence of phytochemicals like flavonoids and tannins which act as antioxidants. This study has demonstrated that <em>B. coriacea </em>crude seed powder, methanolic and aqueous extracts caused no adverse effect on the rat kidney and may be recommended for the management of nephrotoxicity</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 227-235, 2018 (December)</p> Godwin C. Ugwu Chinagorom L. Okanya Jude V. Egbuji Jude I. Okwo Emmanuel I. Nnamonu Joseph E. Eyo ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 227 235 10.3329/dujps.v17i2.39180 Thrombolytic, Membrane Stabilizing and Hypoglycemic Activities of Anethum sowa Linn. https://www.banglajol.info/index.php/JPharma/article/view/39181 <p>The hexane (HE), dichloromethane (DCME), ethyl acetate (EAE) and methanol (ME) extracts of seed and stem of <em>Anethum sowa </em>were subjected to screenings for thrombolytic, membrane stabilizing and hypoglycemic activities. Ethyl acetate extract of stem showed highest thrombolytic activity. In addition, the dichloromethane, ethyl acetate and methanol extract of seed revealed higher percentage (%) of inhibition in hypotonic solution induced hemolysis. In hypoglycemic activity, the dichloromethane and ethyl acetate extracts of stem and seed displayed significant blood glucose lowering effect.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 237-241, 2018 (December)</p> Muhammad Abdullah Al Mansur M Mahboob Ali Siddiqi Koushik Saha ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 237 241 10.3329/dujps.v17i2.39181 Acute Toxicity and Hepatotoxicity Evaluation of Methanol Extract of Root Bark of Calotropis gigantea in Rats https://www.banglajol.info/index.php/JPharma/article/view/39182 <p>Many studies of root extract of <em>Calotropis gigantea </em>have been done to prove its potential as anticancer, antimicrobial, etc. agent <em>C. gigantea </em>plant itself is very easy to grow in tropical countries. However, studies of acute toxicity of <em>C. gigantea </em>root extract has not been performed.The purpose of this research was to know the safety level, the chemical constituents, and the acute toxicity of methanol extract of <em>C. gigantea </em>root bark given orally on <em>Rattus norvegicus </em>in rats. <em>C. gigantea </em>root bark was extracted by using methanol. The methanol extract was suspended in 1% sodium carboxymethyl cellulose (CMC) and administered orally by gavage (1250, 2500 and 5000 mg/kg) in separate groups. On the day of fifteen, all animals were anesthetized and some selected vital organs were excised, weighed and macroscopically examined. The liver was assessed histopathologically. There were no lethal effects, behavioral changes and no significant change in body and organ weights compared to control after the administration of the extracts. Thus, the value of LD<sub>50</sub> for oral administration of methanol extracts from root bark of <em>C. gigantea </em>was larger than 5000 mg/kg. Methanol extract of <em>C. gigantea </em>root bark must be considered safe enough as none of the rats were died along the study. But, it can damage the hepatic cell, if given in higher dose.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 243-250, 2018 (December)</p> Kartini Hasballah Murniana Sarong Renzavaldy Rusly Karina Tantri Vera Dewi Mulia ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 243 250 10.3329/dujps.v17i2.39182 Antinociceptive and Anthelmintic Activities of Leaves of Leea aequata https://www.banglajol.info/index.php/JPharma/article/view/39183 <p>The objective of the study was to evaluate the antinociceptive and anthelmintic activities of the crude methanol extract of leaves of <em>Leea aequata </em>and its fractions. The crude extract and its fractions at 200- and 400-mg/kg bw were subjected to assay for their antinociceptive activity using acetic acid induced writhing and radiant heat tail flicking methods. The ethyl acetate soluble fraction at 400 mg/kg bw induced 40.97% inhibition of writhing in mice while the carbon tetrachloride and chloroform soluble fractions of crude extract at the same dose displayed activity with 40.28% inhibition of writhing as compared to standard diclofenac sodium. The crude extract elongated the reaction time by 57.04% after 30 minute of administration in radiant heat tail flicking method, which suggested the central antinociceptive activity as compared to morphine. The methanol extract of the leaves of <em>L. aequata </em>exhibited profound anthelmintic activity in a dose dependent manner with shortest time of paralysis and death at 100 mg/ml concentration. It caused paralysis of the earthworm <em>Pheretima posthuma </em>at 9.44 min and death at 12.9 min when compared to the standard drug albendazole, which at 10 mg/ml concentration revealed the same at 8.21 minutes and 11.18 minutes, respectively.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 251-255, 2018 (December)</p> Sushanta Halder Nazmus Saqueeb Nazmul Qais ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 251 255 10.3329/dujps.v17i2.39183 Stereospermum suaveolens (Roxb.) DC. Shows Potential in vivo and in vitro Bioactivities https://www.banglajol.info/index.php/JPharma/article/view/39184 <p>The methanol extract of <em>Stereospermum suaveolens </em>(Roxb.) DC was investigated for antidiabetic, antidiarrheal and analgesic activities in Swiss Albino mice. Antidiabetic activity was evaluated by oral glucose tolerance test where the crude extract of <em>S. suaveolens </em>(400 mg/kg b.w.) exhibited 56.10% reduction of blood glucose level as compared to 58.53% by standard glibenclamide (0.1 mg/kg b.w.). In the castor oil-induced diarrhea in mice, the plant extract, at the dose of 400 mg/kg b.w. demonstrated 42.11% reduction of diarrheal feces, while the standard loperamide revealed 57.89% reduction of diarrheal feces. The analgesic activity of <em>S. suaveolens </em>was assessed by both radiant heat tail-flick and acetic acid-induced writhing test. The methanolic extract and different Kupchan fractions of <em>S. suaveolens </em>were also subjected to screening for total phenolic content, DPPH free radical scavenging assay, membrane stabilizing, thrombolytic and antimicrobial activities. In the DPPH assay, the aqueous soluble fraction of methanolic extract revealed highest antioxidant properties with IC50 value of 18.99 μg/ml. The membrane stabilizing activity was assessed by hypotonic solution- and heat-induced methods and was compared with standard acetyl salicylic acid. In hypotonic solution-induced haemolysis, the hexane and carbon tetrachloride soluble fraction inhibited 54.42% and 52.67% haemolysis of RBCs, respectively. On the other hand, in heat-induced haemolysis, the chloroform soluble fraction inhibited the haemolysis of RBC by 57.10% as compared to 72.09% produced by acetyl salicylic acid. In antimicrobial assay by disc diffusion method, only the hexane and carbon tetrachloride soluble fractions demonstrated moderate antimicrobial activity (zone of inhibition = 7.0-15.0 mm) against the test organisms.</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 257-263, 2018 (December)</p> Md Moniruzzaman Md Ruhul Kuddus Mohammad Rashedul Haque AM Sarwaruddin Chowdhury Mohammad A Rashid ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 257 263 10.3329/dujps.v17i2.39184 14-Deoxyandrographolide Isolated from Andrographis paniculata (Burm. f) Nees Growing in Bangladesh and its Antimicrobial Properties https://www.banglajol.info/index.php/JPharma/article/view/39185 <p>Abstract not available</p> <p>Dhaka Univ. J. Pharm. Sci. 17(2): 265-267, 2018 (December)</p> Parisa Tamannur Rashid Muniruddin Ahmed Md Mizanur Rahaman Md Abdul Muhit ##submission.copyrightStatement## 2018-12-04 2018-12-04 17 2 265 267 10.3329/dujps.v17i2.39185