Dhaka University Journal of Pharmaceutical Sciences 2019-07-09T06:51:18+00:00 Prof. Dr. Md. Abdur Rashid Open Journal Systems <p>The Dhaka University Journal of Pharmaceutical Sciences is published by the Faculty of Pharmacy, University of Dhaka, Bangladesh. Full text articles available</p> <p><strong>Journal Metrics</strong><br><a href=";metricName=&amp;sortDirection=ASC&amp;offset=&amp;displayAll=true&amp;sortPerformedState=f&amp;origin=sourceSearch&amp;sortDirectionMOne=&amp;sortDirectionMTwo=&amp;sortDirectionMThree=&amp;metricDisplayIndex=1&amp;scint=1&amp;menu=search&amp;tablin=&amp;searchWithinResultsDefault=t&amp;searchString=&amp;searchOA=&amp;typeFilter=d_j_p_k&amp;subscriptionFilter=s_u&amp;filterActTriggered=f&amp;tabName=searchSources&amp;searchTermsSubmit=&amp;searchType=issn&amp;searchTerms=1816-1820" target="_blank" rel="noopener">CiteScore Tracker 2017</a>: Updated on January 28, 2019&nbsp; <img src="/public/site/images/admin/index11.jpg"></p> <p><a href="" target="_blank" rel="noopener">ResearchGate Journal Impact</a>&nbsp;(2015): 0.52 <img src="/public/site/images/admin/Research_Gate1.jpg"></p> <p><a href=";tip=sid" target="_blank" rel="noopener">Scimago Journal Rank</a> (2017): 0.18 <img src="/public/site/images/admin/j.jpg" width="155" height="156"></p> <p><a href="" target="_blank" rel="noopener">MIAR Analysis</a> : Updated on January 28, 2019 <img src="/public/site/images/admin/MIAR.jpg"></p> Development and Optimization of Acyclovir Loaded Mucoadhesive Microspheres by Box – Behnken Design 2019-07-09T06:32:06+00:00 James Regun Karmoker Ikramul Hasan Nusrat Ahmed Mohammad Saifuddin Md Selim Reza <p>The grail of the study was to design, develop and characterize sustained release mucoadhesive microspheres of acyclovir and to optimize the drug release profile using response surface methodology by applying Box–Behnken design (BBD) which was equipped with three levels and three factors. Microspheres were prepared from Methocel K15M and Ethocel Standard 45 Premium using the emulsification solvent evaporation technique. The independent factors were the amounts of Methocel K15M (X<sub>1</sub>), amount of Ethocel Standard 45 Premium (X<sub>2</sub>), and RPM (X<sub>3</sub>). The dependent variables were cumulative percentage drug release (CDR) at 8 hour (Y<sub>1</sub>), bond strength (Y<sub>2</sub>), and swelling at 4 hour (Y<sub>3</sub>). To understand the effects of different factor level combinations on the responses, various response surface graphs and contour plots were prepared. Predicted values and experimental values for optimized formulation (X<sub>1</sub> = 600 mg, X<sub>2</sub> = 500 mg, and X<sub>3</sub> = 336.57) was found to be in close agreement.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 1-2, 2019 (June)</p> 2019-05-16T00:00:00+00:00 ##submission.copyrightStatement## Secondary Metabolites and Antioxidant Potentials of Axis Sea Bamboo (Isis hippuris) 2019-07-09T06:32:10+00:00 Mohammad Sayuti Widya Dwi Rukmi Putri - Yunianta <p>Secondary metabolites of the axis section (modulla skeleton) of sea bamboo (<em>Isis hippuris</em>) were identified by GC-MS. The dominant compounds using GC-MS in the ethanol fraction were hexanedioic acid (11.85%), acetamide (9.46%), <em>n</em>-hexadecanoic acid (9.22%) and thiosulfuric acid (7.22%). On the other side, the dominant compounds of the test results in the ethyl acetate fraction were 7-oxabicyclo[4.1.0] heptanes (28.27%), 1,2- benzene dicarboxylic acid (14.77%), <em>cis</em>-8-(N-pyrrolidyl)-(2,2,5,5-tetradeutero)bicyclo[4.3.0]nona-3,7-diene (9.98%), hexahydropyridine (7,86%). The dominant compounds of the <em>n</em>-hexane fraction were hexanedioic acid (41.99%), azetidine with a peak number of 40 (9.98%), 1-octadecene (8,36%). Antioxidant activities were also evaluated by DPPH scavenging assay. The ethanol, <em>n</em>-hexane and ethyl acetate fractions showed total flavonoid contents of 7.86% ± 0.12, 12.97% ± 0.36, 1.88% ± 0.26, respectively and the IC50 in the antioxidant assay were 480.25±74,74, 469.50±19,13, 3221.07±138,69 respectively.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 13-20, 2019 (June)</p> 2019-05-16T00:00:00+00:00 ##submission.copyrightStatement## Characterization and Disintegrant Potential of Phosphorylated Tiger Nut (Cyperus esculentus) Starch in Immediate Release Ibuprofen Tablet Formulation 2019-07-09T06:32:14+00:00 Onyinye D Onwuatuegwu Chukwuemeka P Azubuike Sinmisola Aloko Modupe O Ologunagba Cecilia I Igwilo <p>The study was aimed at evaluating the physicochemical properties of phosphorylated tiger nut starch (TNP) and its disintegrant properties in immediate release ibuprofen tablets. Native tiger nut starch (TNS) was modified by phosphorylation with disodium hydrogen orthophosphate at 130<sup>o</sup>C and its physicochemical properties were evaluated. Ibuprofen tablets were formulated with TNP and sodium starch glycolate (SSG) at concentrations of 5.0, 7.5, 10.0 and 15.0% as disintegrants. Phosphorylation of TNS led to improved flow properties and swelling and hydration capacities among other changes in the physicochemical properties. TNP had comparable properties with SSG. FTIR study confirmed modification and also showed that TNP is compatible with ibuprofen powder. Ibuprofen tablets produced with TNP as disintegrant had acceptable tablet properties comparable to those produced with SSG. The disintegrant potential improved with increased concentration of TNP. The results indicate that TNP has a promising disintegrant potential in tablet formulations.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 21-29, 2019 (June)</p> 2019-05-16T00:00:00+00:00 ##submission.copyrightStatement## Cholinesterase and Glycation Inhibition Assay of Several Metabolites Obtained from Plant and Fungi 2019-07-09T06:32:17+00:00 Rashedul Islam Mohiminul Adib Monira Ahsan Md Mustafizur Rahman Md Abdul Mazid <p>Cholinesterase inhibition is one of the major strategies to develop better quality anti-Alzheimer’s drug, while inhibition of glycation pathway is a useful therapeutic strategy to treat diabetic complications. This study was designed to evaluate the anti-acetylcholinesterase, anti-butyrylcholinesterase and anti-glycation activities of 8 secondary metabolites namely, RS-8, RS-22C (arborinine), CL-1 (betulinic acid), CL-2 (ursolic acid), CCL-1 (cajaninstilbene acid)<em>, </em>CP-1 (beta-sitosterol)<em>, </em>HS-1 (anhydrofusarubin) and HS-2 (methylether of fusarubin). It was found that RS-8 and arborinine showed promising activity in inhibiting acetylcholinesterase with IC<sub>50</sub> values of 24.40 ± 0.39 and 13.14 ± 0.07, respectively. Anhydrofusarubin and methyl ether of fusarubin exhibited moderate activities with IC<sub>50</sub> values of 47.82 ± 0.54 and 44.58 ± 0.8. Arborinine and cajaninstilbene acid potentially inhibited butyrylcholinesterase with IC50 values of 26.34 ± 0.31 and 25.82 ± 0.34, respectively, but other metabolites did not show such inhibitory activities. Inhibition of glycation process was evaluated by bovine serum albumin assay but none of the metabolites significantly inhibited the glycation pathway.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 31-38, 2019 (June)</p> 2019-05-16T00:00:00+00:00 ##submission.copyrightStatement## Pharmacoeconomic Analysis of Alcohol-Dependent Patients Drug Therapy 2019-07-09T06:32:19+00:00 Larisa B Vaskova Elena A Maksimkina Manuk A Arabyan <p>Methodologic approach to the pharmacoeconomic evaluation of the drug therapy for alcoholdependent patients in an inpatient facility in real clinical practice was proposed by the authors. The aim of the study was to perform a pharmacoeconomic analysis of the disease treatment cost in alcohol-dependent patients according to their primary diagnosis in an inpatient facility.During the period from 2015 to 2016, the authors analyzed 120 medical histories of patients with primary diagnosis F.10.2 (Alcohol dependence) – disease-related group (DRG) №1 and F.10.3 (Alcohol withdrawal syndrome) – DRG №2. In total, in 2015 there were 27 drugs from 15 PT groups indicated and in 2016 – 36 drugs from 16 PT groups. It was established that in 2015 the main budget share was spent on PTG antiepileptics and vitamins (68.14 USD or 21.24% and 59.23 USD or 18.46% of the total sun, respectively), and in 2016 - on PTG anxiolytics and antidepressants (99.41 USD or 16.67%).</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 39-42, 2019 (June)</p> 2019-05-16T00:00:00+00:00 ##submission.copyrightStatement## In vitro Pharmaceutical Equivalence Study of Three Brands of Atenolol Tablets Available in Bangladesh 2019-07-09T06:32:20+00:00 Rehana Begum Md Zakir Sultan Jakir Ahmed Chowdhury Md Shah Amran <p>The aim of the present work was to assess the pharmaceutical equivalence of three brands of atenolol (50 mg) tablets available in Bangladesh using <em>in vitro </em>dissolution study. The dissolution study was carried out using the paddle apparatus according to the guidelines of United States Pharmacopoeia (USP). The dissolution profiles of three locally manufactured atenolol tablets were determined and compared with the dissolution profile of atenolol tablet from innovator’s company. All samples attained more than 85% dissolution within 10 minutes. Mean dissolution values were employed to estimate difference factor (f1) and similarity factor (f2). Difference factor (f1) and similarity factor (f2) were used to assess <em>in vitro </em>bio-equivalency among the three brands. Other general quality assessment parameters such as hardness, friability and disintegration time were also determined. All brands complied with the official specifications for hardness, friability and disintegration time. The study indicated that all brands can be prescribed interchangeably.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 43-48, 2019 (June)</p> 2019-05-16T00:00:00+00:00 ##submission.copyrightStatement## Alternanthera bicolor Produces Hypoglycemic Effect in Alloxan-Induced Diabetic Mice through its Antioxidant Activity 2019-07-09T06:32:21+00:00 Kousik Ahmed Khan Manik Zahan Fatema Zohura Talukder Riaz Uddin Manik Chandra Shill Hemayet Hossain Lutfun Nahar Satyajit Dey Sarker Md Ashraful Alam Hasan Mahmud Reza <p>The current investigation was carried out to evaluate the antioxidant properties of ethanolic extract of <em>Alternanthera bicolor </em>and to assess the potential hypoglycemic effect of the extract in alloxan-induced diabetic mice. HPLC-DAD method was used to determine polyphenolic compounds present in the extract. Different <em>in vitro </em>assays (i.e. DPPH radical scavenging activity test, reducing power test, NO radical inhibition assay and scavenging of hydrogen peroxide) were used to determine the antioxidant potential of the plant. Antidiabetic activity was evaluated in alloxan-induced diabetic mice by glucose tolerance test and standard biochemical analyses. HPLC-DAD analysis of the extract confirmed the presence of (+)-catechin hydrate, caffeic acid, quercetin and kaempferol. <em>A. bicolor </em>showed potent antioxidant activities in DPPH radical, hydrogen peroxide and nitric oxide scavenging assays. Moreover, <em>A. bicolor </em>showed potent reducing power and dose-dependent increment of total antioxidant capacity. Furthermore, the plant showed potent hypoglycemic activities in alloxan-induced diabetic mice. Ethanolic extract of the plant at doses of 200- and 400-mg/kg body weight (administered orally for 3 weeks) significantly decreased the elevated levels of blood glucose, lipid peroxidation product TBARS, hydroperoxides and nitric oxide in experimental animals. Apart from these activities, the ethanolic extract of the plant restored the reduced catalase function in liver. From this study, we can conclude that ethanolic extract of <em>A. bicolor </em>exhibited hypoglycemic and antioxidant activities in alloxan-induced diabetic mice.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 49-60, 2019 (June)</p> 2019-05-16T00:00:00+00:00 ##submission.copyrightStatement## Enantiomeric Determination of Carvedilol by a Newly Developed and Validated Chiral HPLC Method 2019-07-09T06:32:23+00:00 Asma Rahman Mohammad Rashedul Haque Md Zakir Sultan M Muhibur Rahman Mohammad A Rashid <p>A new simple, selective, linear and accurate chiral HPLC method for assay of carvedilol enantiomers was developed with immobilized cellulose chiral stationary phases under normal-phase mode using hexane: isopropyl alcohol: diethyl amine: acetic acid (40: 60: 0.7: 0.3, v/v) as the mobile phase at a flow rate of 1.0 mL/min and detection at 220 nm. The method was validated for linearity, precision, accuracy, ruggedness, robustness and stability as per the guidelines of USP and ICH. The regression coefficients (r<sup>2</sup>) of the linearity were found to be 0.999 for both S- and R- carvedilol, detection limit (LOD) were 1.67 and 1.78 μg/mL and quantitation limit were also found to be 5.06 and 5.41 μg/mL for S- and R- carvedilol, respectively. The average percentage of recovery was found to be 99.59% to 100.83% for S- and 98.42% to 100.18% for R- carvedilol, respectively. The new method can be used for enantiomeric separation and estimation of carvedilol in pharmaceutical dosage forms without any interference.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 61-68, 2019 (June)</p> 2019-05-16T00:00:00+00:00 ##submission.copyrightStatement## Phytochemical Evaluation of Extracts and GC-MS analysis of oil from Monodora myristica Seed 2019-07-09T06:32:24+00:00 Wilfred O Obonga Edwin O Omeje Charles O Nnadi Wilson G Ocheme <p><em>Monodora myristica </em>is one of the plants used extensively in folkloric medicine across West Africa. Despite the ethnopharmacological relevance of the plant, the phytochemical details, especially of the oil-rich seed, have not been completely elucidated. This study was designed to ascertain the phytochemical constituents of extracts of <em>M</em>. <em>myristica </em>seed oil and characterize the oil constituent using gas chromatography-mass spectrometry. The phytochemical screening was done using standard methods and the oil was characterized by GC-MS analysis. The result of the phytochemical screening showed the presence of terpenoids, sterols, saponins, tannins, flavonoids and cardiac glycoside in variable quantities. The GC-MS analysis revealed huge presence of fatty acids, terpenoids and other related compounds. The major compounds found in the oil of the seeds were <em>o-</em>cymene (<strong>2)</strong>, γ-terpinene (<strong>3</strong>), nopinane (<strong>4</strong>), limonene (<strong>5</strong>), carvotanacetone (<strong>6</strong>), β-pinene (<strong>7</strong>), aromadendrene (<strong>9</strong>), germacrene (<strong>11</strong>), α-amorphene (<strong>13</strong>) and copaene (<strong>14</strong>). The rich phytoconstituents present in the seed oil could be the basis of its acclaimed ethnomedicinal uses across West Africa and beyond.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 69-73, 2019 (June)</p> 2019-06-24T00:00:00+00:00 ##submission.copyrightStatement## Syntheses, Characterization and Biological Evaluation of a Series of 2-Phenylamino-5-(2-Chlorophenyl)-1,3,4-Oxadiazole Derivatives 2019-07-09T06:32:25+00:00 S Kumar PK Srivastava <p>Electrochemical synthesis of 2-phenylamino-5-(2-chlorophenyl)-1,3,4-oxadiazoles have been carried out in good yields at platinum electrode through the electrochemical oxidation of acyl thiosemicarbazide at room temperature in acetic acid. Two platinum electrodes in the form of square plates were used as working as well as counter electrode and saturated calomel electrode was used as reference electrode. The structure of the compounds was confirmed by IR, NMR, mass spectral and elemental analyses. The antibacterial activity of the derivatives was also assessed and compared with data against a series of Gram-positive <em>Klebsiella pneumoniae</em>, <em>Escherichia coli </em>and Gramnegative bacteria <em>Streptococcus aureus </em>and <em>Bascillus subtilis</em>. The antifungal activity was assessed against the fungal strain <em>Aspergillus niger, Crysosporium pannical, Pellicularia solmanicolor </em>and <em>Candida albicans </em>and compared against the standard antifungal drug Griesvofulvin.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 75-83, 2019 (June)</p> 2019-06-24T00:00:00+00:00 ##submission.copyrightStatement## Determination of Amino Acid Composition in the Harpagophytum procumbens Root 2019-07-09T06:32:26+00:00 AI Kriukova IM Vladymyrova OL Levashova TS Tishakova <p>The amino acid composition of the roots of <em>Harpagophytum procumbens </em>was investigated by the method of high performance liquid chromatography (HPLC) with preliminary derivatization. Sixteen free and thirteen bound amino acids were quantitatively determined. The content of protein-bound amino acids was calculated.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 85-91, 2019 (June)</p> 2019-06-24T00:00:00+00:00 ##submission.copyrightStatement## Cycloartane and Stigmastane Type Triterpenoids from Pothos scandens Inhibit Estradiol (E2) Induced Proliferations in Breast Cancer Cells 2019-07-09T06:32:27+00:00 Md Abdul Muhit Kaoru Umehara Nahid Sharmin Hiroshi Noguchi <p>Four cycloartane type triterpenoids and three stigmastane type steroids were isolated from the methanolic extract of stem and root part of Pothos scandens L. (Araceae), a Bangladeshi medicinal plant by high performance liquid chromatographic technique. The compounds were characterized as 24-methylenecycloartanol (1), 24-methylenecycloartenone (2), 24-en-cycloartenone (3), 24-methylenecycloartanyl ferulate (4), stigmast-4-en-3-one (5), stigmast-4,22-diene-3-one (6) and β-sitosterol glucoside (7) through extensive 1D and 2D NMR spectroscopic studies. All the isolates were evaluated for their estrogenic/antiestrogenic activity using the estrogen-responsive breast cancer cell lines, MCF-7 and T47D. The results showed that all the compounds possess mild to strong antiestrogenic activity in both cell lines which was compared to positive control tamoxifen. 24- Methylenecycloartanol (1), which contains a hydroxyl group in its C-3 position, inhibited 90% of estradiol (E2)- induced cell proliferation in MCF-7 and T47D cell lines at a concentration of 0.01 μM only. 24- Methylenecycloartanyl ferulate (4) and stigmast-4,22-diene-3-one (6) showed 90% of estradiol (E2)-induced cell proliferation in T47D cell only at a concentration of 0.01 μM whereas 10.0 μM was required for 24- methylenecycloartanyl ferulate (4) for the same activity in MCF-7 cells. This is the first report of isolation of these compounds from the plant along with their antiestrogenic property.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 93-102, 2019 (June)</p> 2019-06-24T00:00:00+00:00 ##submission.copyrightStatement## Application of Statistical Design to Assess the Critical Process Parameters of Ethanol Injection Method for the Preparation of Liposomes 2019-07-09T06:32:29+00:00 Sayani Bhattacharyya Bharani S Sogali <p>In the present study custom screening design was employed to observe the effect of four critical process parameters on particle size and polydispersity index of the liposomal formulation made by ethanol injection method. The four process parameters selected were lipid ratio, rate of injection, phase volume ratio and rotational speed of magnetic stirring. Eight different liposomal formulations were prepared using the design. The formulations were subjected to particle size analysis. The analysis was done at a significance level p&lt;0.05 and found that the process parameters had significant effect on the particle size and polydispersity index of the formulations. The design was optimized for the individual responses with an overall desirability of more than 50%. Three batches of liposomes were formulated at optimized process parameters which matched the target as predicted by the design. Therefore, it can be concluded that the design was effective in production of nano sized stable monodisperse liposomes by ethanol injection method.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 103-111, 2019 (June)</p> 2019-06-24T00:00:00+00:00 ##submission.copyrightStatement## Clinical Interventions of Critical Care Pharmacist in the Therapeutic Management of Critically Ill Patients: a Retrospective Study in Bangladesh 2019-07-09T06:32:30+00:00 Md Jahidul Hasan Raihan Rabbani Sitesh C Bachar <p>Critically ill patients at ICU are treated with poly pharmacy and conservative drug management is necessary for ensuring drugs’ safety and accuracy. The objective of this study was to analyze the qualitative intervention of critical care pharmacists (CCP) in critically ill patients’ effective medication management. This was a 6 months long observational study. All the provided suggestions of CCP were categorized into- A (drug-drug interaction), B (addition of new drug therapies), C (rational dosing of antibiotics), D (acceleration or deceleration of the doses) and E (adverse drug reaction). Out of total CCP’s 650 suggestions, 566 (87.08%) suggestions were accepted by doctors and modified the therapies, accordingly. CCP being a part of ICU’s multi professional team contribute the professional roles in generating safe, appropriate and quality prescriptions, which finally turns into quality pharmacotherapy for critically ill patients at ICU.</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 113-119, 2019 (June)</p> 2019-06-24T00:00:00+00:00 ##submission.copyrightStatement## Total Phenolic Content, Membrane Stabilization and Thrombolytic Activities of Corypha taliera (Arecaceae) Flowers 2019-07-09T06:32:08+00:00 Akhtaruzzaman Chowdhury Md Shafiullah Shajib Faiza Tahia Md Ashraful Alam Mohammad A Rashid <p>Abstract not available</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 121-123, 2019 (June)</p> 2019-05-16T00:00:00+00:00 ##submission.copyrightStatement## Development and Validation of RP-HPLC Method for Analysis of Rabeprazole Sodium 2019-07-09T06:32:12+00:00 Sumaya Mahmud Sharna Mehedi Hasan Shuvo Syef Ferdaus Sabiha Chowdhury <p>Abstract not available</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 125-128, 2019 (June)</p> 2019-05-16T00:00:00+00:00 ##submission.copyrightStatement## Preparation and In vitro Evaluation of Orally Disintegrating Tablets (ODTs) of Alprazolam 2019-07-09T06:32:16+00:00 Nusrat Islam Chaity Sabrina Rahman Archie Nusrat Ahmed <p>Abstract not available</p> <p>Dhaka Univ. J. Pharm. Sci. 18(1): 129-133, 2019 (June)</p> 2019-05-16T00:00:00+00:00 ##submission.copyrightStatement##