https://www.banglajol.info/index.php/JPharma/issue/feed Dhaka University Journal of Pharmaceutical Sciences 2021-06-20T02:05:59+00:00 Prof. Dr. Md. Abdur Rashid dujps_du@yahoo.com Open Journal Systems <p>The Dhaka University Journal of Pharmaceutical Sciences is published by the Faculty of Pharmacy, University of Dhaka, Bangladesh. Full text articles available</p> <p>&nbsp;</p> https://www.banglajol.info/index.php/JPharma/article/view/50852 Evaluation of Current Status of Newly Established Model Pharmacies in Bangladesh 2021-06-20T02:05:37+00:00 Shadhan Kumar Mondal abmfaroque@yahoo.com Sabiha Chowdhury abmfaroque@yahoo.com Amlan Ganguly abmfaroque@yahoo.com ABM Faroque abmfaroque@yahoo.com <p>Pharmaceutical sector of Bangladesh has developed profoundly after promulgation of the Drugs (Control) Ordinance, 1982. However, the health sector has not been equally developed because of lack of wellequipped drug management system and much needed patient counseling. The presence of adulterated, counterfeit and substandard drugs and the sale of drugs at high prices than the maximum retail price have also been the major problems here. The recent introduction of model pharmacies is supposed to be a hope for the people to get safe medicines at a reasonable cost. The aim of the present study was to find out the current scenario of model pharmacies in Bangladesh and to propose modern and alternative systems that could be applied in model pharmacies for better healthcare management and patient compliance. Thus, the current status of model pharmacies of Bangladesh has been evaluated using a survey-based analysis which utilized a pre-set questionnaire. The survey was conducted on 90 model pharmacies in seven districts of Bangladesh (Level 1 categorized by the Directorate General of Drug Administration, Government of the People’s Republic of Bangladesh). The results revealed that the infrastructure of the model pharmacies should be improved further. Only 33% of the model pharmacies have sitting facilities and 51% of them have washroom facilities for the waiting patients. It was found that despite all the model pharmacies (100%) should have at least 1 A-grade pharmacist in each of them, i.e. a pharmacy graduate registered with the Pharmacy Council of Bangladesh under the Pharmacy Ordinance 1976, but pharmacists were found to be present in only 26% of pharmacies during the visit. Amongst the pharmacists, 98% showed satisfaction with the decision of compulsory engagement of A-grade pharmacists in all the model pharmacies. Defying the obligatory provisions, only 38% model of pharmacies keep the required records of sold drugs. It was pleasing to observe that no physician’s sample of medicines was sold in any model pharmacies. The medicines storage facilities in controlled temperature was found in all the model pharmacies. But the A-grade pharmacists were not available in the pharmacies during holidays. It is opined that modern and ICT based techniques can be applied to modify the model pharmacies for better patient care and patient management.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 1-10, 2021 (June)</p> 2021-06-20T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/50853 Formulation of Non-effervescent Floating Dosage Form of Metronidazole using Sintering and Sublimation Technique 2021-06-20T02:05:39+00:00 Airemwen Collins Ovenseri collins.airemwen@uniben.edu Uhumwangho Uwumagbe Michael collins.airemwen@uniben.edu <p>The aim of this study was to formulate a novel non-effervescent floating dosage form of metronidazole using the sublimation and sintering technique. Granules were formulated using the wet granulation technique. Ammonium bicarbonate (30% w/w) was incorporated as the sublimating agent. The granules were characterized for micromeritic properties. Thereafter, the granules were compressed using a single punch tableting machine and the physicotechnical properties were evaluated. The metronidazole tablet was then sintered at 70oC for 12 h. All granules were free flowing and compressible. The metronidazole tablets had no floating lag time showing that tablets floated instantaneously. FTIR and DSC studies showed that metronidazole and the excipients used in the formulation were compatible. <em>Azadirachta indica </em>gum was used in the formulation of non-effervescent floating dosage form of metronidazole using sublimation and sintering technique which is beneficial in sustained release formulations.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 11-17, 2021 (June)</p> 2021-06-20T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/50854 Formulation Development and Evaluation of Mouth Dissolving Tablet of Aspirin by Using QbD Approach 2021-06-20T02:05:40+00:00 Nilima A Thombre nilimathombre@gmail.com Pradeep S Ahire nilimathombre@gmail.com Sanjay J Kshirsagar nilimathombre@gmail.com <p><strong>In </strong>the current investigations, mouth dissolving tablets (MDT) were developed by applying quality by design (QbD) approach. Direct compression method was applied for the preparation of MDT containing aspirin using 3<sup>2</sup> factorial design with quantity of drug, microcrystalline cellulose (MCC) and crosscarmellose sodium (CCS) as dependant variables. MCC and CCS were used as superdisintegrants. Sodium stearyl fumarate was used as lubricant. Developed MDT were evaluated for characteristics like hardness, friability, disintegration time (DT) and <em>in vitro </em>drug release . Design Expert 11.0 described adequately impact of selected variables (MCC and CCS) at various levels for response under study (DT and friability). The optimized batch showed disintegration time of 15-28 secs, friability within 1% and <em>in vitro </em>drug release of 75-98% after 30 mins, respectively. The present study of experimental design revealed that MCC and CCS are fruitful at low concentration to develop the optimized formulation. As per the results obtained from the experiments, it can be concluded that QbD is an effective and efficient approach for the development of quality into MDT with the application of QTPP, risk assessment and critical quality attributes (CQA).</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 19-29, 2021 (June)</p> 2021-06-20T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/50855 Characterization of Chitosan Obtained from Snail Shell (Achatina fulica) and its Excipient Potentials in Metronidazole Tablet Formulation 2021-06-20T02:05:42+00:00 Oluyemisi Adebowale Bamiro bamroy67@yahoo.co.uk Aishat Oyinkansola Salisu bamroy67@yahoo.co.uk Ese Mary Iyere bamroy67@yahoo.co.uk Olatundun Atoyegbe bamroy67@yahoo.co.uk Olutayo Ademola Adeleye bamroy67@yahoo.co.uk Lateef Gbenga Bakre bamroy67@yahoo.co.uk <p>The aim of the work was to characterize chitosan extracted from snail shell and evaluate its use as a disintegrant and binder in metronidazole tablet formulation in comparison with standard chitosan (SC). The mechanical properties were assessed using crushing strength and friability, while the release properties were assessed using disintegration and dissolution times. The extracted chitosan (EC) was crystalline in nature and the scanning electron microscopy (SEM) showed polygonal particles with rough surface. The moisture and swelling capacity was 1.80% and 15.00%, respectively. The densities and flow properties were significantly (p&lt;0.05) higher than those of the SC. As a binder, the crushing strength of formulations containing EC was higher than SC, but both formulation failed friability test. There was significant difference between the disintegration times of the metronidazole formulations containing EC and SC as a disintegrant. The result showed that EC is more effective as a binder in tablet formulations.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 31-39, 2021 (June)</p> 2021-06-20T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/50856 A Study on the National Drug Policies of Bangladesh to Ensure Health for All 2021-06-20T02:05:43+00:00 Md Aknur Rahman amranms@du.ac.bd Md Riaz Hossain amranms@du.ac.bd Md Aslam Hossain amranms@du.ac.bd Md Shah Amran amranms@du.ac.bd <p>Bangladesh approved the proposal for a National Drug Policy on May 29, 1982. We know that such drug policies are developed gradually over a period of time and may contain a lot of comprehensive documents. But in Bangladesh, the expert committee worked out the policy, based on 16 standards within 15 days. This vital document, almost unchanged, was made a law on 12 June 1982. A few years later, it can be observed that despite opposition from many concerns, the output of essential drugs has increased from about 30 to about 80 percent, prices have in almost all cases gone down considerably, the domestic industry has grown rapidly, the quality of its production has increased dramatically, and people’s awareness about quality medicines has been steadily growing. The World Health Organization (WHO) has stressed the need of a formulated drug policy in every country of the world in 1986. Bangladesh responded very early to this respect. Subsequently, two more national drug policies were promulgated in 2005 and 2016 respectively. Experience over the decades has shown that the said policies could not fulfill the declared objective of ensuring health for all. Our aim is to describe some of the lacunae for which total implementation of drug policy is still struggling. To find the root causes, a total of five hundred volunteers were surveyed by supplying a questionnaire on drug policy. It was observed that most of the participants opined that the incumbent government needs to be more stringent to implement the drug policy into reality by utilizing the public servants and public sectors, especially health personnel to ensure health for all.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 41-48, 2021 (June)</p> 2021-06-20T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/54032 Physicochemical Characteristics and HPLC Determination of Alpha-Tocopherol in Eighteen Edible Vegetable Oils Marketed in Nigeria 2021-06-20T02:05:45+00:00 Olufunmilayo Ebunoluwa Adejumo funmijumo@yahoo.co.uk Elizabeth Ayodele Popoola funmijumo@yahoo.co.uk Oluyemisi Adebowale Bamiro funmijumo@yahoo.co.uk John Olabanji Daodu funmijumo@yahoo.co.uk Olatunde James Olaitan funmijumo@yahoo.co.uk <p>Eighteen brands of vegetable oils available in the local market were extracted with <em>n</em>-hexane before analysis for alpha-tocopherol by RP-HPLC method. The chromatographic separation occurred isocratically with methanol-water [96:4%v/v] at 0.9 ml/min flow rate. Tocopheryl acetate was the internal standard and alpha-tocopherol was eluted at 7.87 min. Free fatty acids value [FFAVs], peroxide value [PV], iodine value [IV] and saponification values [SV] were determined as quality parameters. Calibration curve was linear [r2 0.9969] and the method was precise with relative standard deviation of 0.35% and mean recovery, 87.39%. Alpha-tocopherol concentration ranged from 0-9.22 mg/100g with the highest in Tropical sunflower oil [9.22 mg/100g] and the lowest [1.16 mg/100g] in Laziz oil. Alpha-tocopherol was not detected in unbranded, local palm oil. The calculated percentage daily value [% DV] of vitamin E ranged from 0- 8.60%. Significant difference [p&lt;0.05] between % DV and recommended dietary allowance [RDA] of vitamin E was observed. FFAs and PV ranged from 0.11-0.74% and 0.99-11.55 meq/kg while IV and SV ranged from 26.71-37.03 g/100g and 4.14-43.68 mg KOH/g, respectively. Seventeen samples [94%] were found to be within the acceptable limits while one [6%], failed for both quality parameters and α-tocopherol test. Strict regulatory control is advocated for these oils to safeguard the public health.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 49-57, 2021 (June)</p> 2021-06-14T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/54033 Evaluation of Body Weight, Serum Lipid Profile, Glucose Homeostasis, Oxidative Stress and Hepatic Function in Healthy Mice Fed With Zinc Sulphate Supplementation 2021-06-20T02:05:46+00:00 Md Atiqur Rahman pharmasalim@nstu.edu.bd Mohammad Tohidul Amin pharmasalim@nstu.edu.bd Sayema Arefin pharmasalim@nstu.edu.bd Dipty Rani Bhowmik pharmasalim@nstu.edu.bd Md Abdur Rahman Ripon pharmasalim@nstu.edu.bd Mohammad Salim Hossain pharmasalim@nstu.edu.bd <p>The present research study was designed to evaluate the effect of zinc supplementation on body weight, serum triglyceride, cholesterol, glucose homeostasis, oxidative stress, and hepatic function in mice. Mice were treated with zinc sulphate at an equivalent weight of 6.5 mg/kg-body weight elemental zinc for four weeks. Bodyweight, serum glucose, triglyceride, cholesterol, serum MDA, nitric oxide, vitamin C, and hepatic enzymes level were determined at the end of the study period. Data from this study showed that supplementation with zinc in mice maintained a balanced blood glucose homeostasis throughout the experimental period. Moreover, treatment with zinc showed a significant (p &lt;0.05) decrease in serum triglyceride and cholesterol level along with a decrease in the body weight compared to control. Treatment with zinc significantly attenuated the rate of lipid peroxidation whereas increased the level of vitamin C and NO level. The protective effect of zinc on liver activity was observed. Treatment with zinc showed a strong negative association with serum total cholesterol (r= -0.934, p = 0.02), triglycerides level (r= -0.709, p = 0.05), and body weight (r= -0.899, p = 0.01). The present findings demonstrate that zinc supplementation can be helpful to maintain a glucose homeostasis, ameliorate hyperlipidemia, oxidative stress, and liver dysfunction. Therefore, zinc supplementation can be suggested to alleviate diseases associated with metabolic syndrome and oxidative stress.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 59-66, 2021 (June)</p> 2021-06-14T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/54034 Physicochemical Characterization of Artemether-Entrapped Solid Lipid Microparticles Prepared from Templated- Compritol and Capra hircus (Goat Fat) Homolipid 2021-06-20T02:05:48+00:00 Petra Obioma Nnamani chimafrankduff@yahoo.com Franklin Chimaobi Kenechukwu chimafrankduff@yahoo.com Chinekwu Sheridan Nwagwu chimafrankduff@yahoo.com Onyinye Okoye chimafrankduff@yahoo.com Anthony Amaechi Attama chimafrankduff@yahoo.com <p>The purpose of this study was to formulate and evaluate the physicochemical properties of artemetherloaded solid lipid microparticles (SLM) prepared from templated-compritol 888<sup>®</sup>ATO and <em>Capra hircus </em>(goat fat) homolipid. Various ratios of compritol 888<sup>®</sup>ATO, goat fat and Phospholipon<sup>®</sup> 90G were used to prepare the templated lipid matrices and characterized by differential scanning calorimetry (DSC). Plain and artemether-loaded SLM (0, 1.0, 3.0 and 5.0% drug) were prepared by melt-homogenization. The SLM were characterized regarding the compatibility by DSC, morphology and particle size by polarized light microscopy (PLM), encapsulation efficiency (EE%), <em>in vitro </em>release in simulated gastric fluid (SGF, pH 1.2), simulated intestinal fluid (SIF, pH 7.2) and alcoholic buffer (pH 3.6), and time-resolved pH-dependent stability. Stable, smooth and mostly spherical SLM with particle sizes in the range 18.77-43.79 μm and EE% ranging from 62.22% to 99.05% were obtained. DSC results showed the compatibility of drug and the formulation excipients as well as the stability of artemether in the developed SLM. Results showed significantly (p&lt;0.05) higher drug release (88.25%) in alcoholic buffer than in SIF and SGF. By implication, incorporation of alcohol in the formulation would be a practical approach to improve artemether bioavailability from the SLM. This study has shown that the physicochemical properties of artemether were improved by SLM based on templated-compritol 888<sup>®</sup>ATO and goat fat.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 67-80, 2021 (June)</p> 2021-06-14T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/54035 Evaluation of Anti-Microbial Activity and Proximate Composition of Alkaloids from Vitex doniana Seed 2021-06-20T02:05:50+00:00 Theophilus K Udeani charles.nnadi@unn.edu.ng Linus O Ugwu charles.nnadi@unn.edu.ng Charles O Nnadi charles.nnadi@unn.edu.ng Chukwugozie N Okwuosa charles.nnadi@unn.edu.ng <p>The study investigated the <em>in vitro </em>anti-microbial activity of crude alkaloids from the seeds of <em>Vitex doniana </em>against some selected bacteria and fungal strains. Agar well diffusion method was used to test the antimicrobial activity using ciprofloxacin and ketoconazole as controls. Phytochemical and proximate analyses were carried out by standard methods. The crude extract showed the presence of alkaloids, tannins and resins in abundance. Proximate analysis indicated fat/oil (11±0.73 %), protein (0.26±0.02 %), moisture (0.21±0.01 %), ash (5.0±0.22 %) and carbohydrate (6.42±0.28 %). The antimicrobial study indicated that the crude extract was more effective against <em>Pseudomonas aeruginosa </em>(MIC 3.84 mg/ml) and <em>Salmonella typhi </em>(MIC 3.84 mg/ml) and other Gram-positive bacteria. The crude alkaloids generally showed lower activity in case of Gram-negative (MIC <em>Salmonella typhi </em>4.20 mg/ml) than in Gram-positive bacteria (MIC <em>Staphylococcus aureus </em>2.52 mg/ml). Surprisingly, the crude alkaloids from the seeds, in addition to improved activity against all the bacteria strains, showed significant activity against <em>Candida albicans </em>(MIC 1.18 mg/mL. <em>V. doniana </em>seed extract was found to be potent against some clinical strains of both Gram-positive and negative bacteria but not fungi; however, its alkaloids has promising antifungal activity.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 81-86, 2021 (June)</p> 2021-06-14T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/54036 Isolation and Characterization of Multidrug Resistant Enterobacteriaceae in Urine Sample of Patients Suffering from Urinary Tract Infection with Diabetes and Nephropathy 2021-06-20T02:05:51+00:00 Nazmul Ahsan nahsan@du.ac.bd Monzilur Rahman nahsan@du.ac.bd Md Nazrul Islam nahsan@du.ac.bd Anwarul Azim Akhand nahsan@du.ac.bd <p>Multidrug-resistant (MDR) organisms are spreading widely and becoming an issue of utmost importance to deal with. In the current study, ten urine samples from diabetic patients suffering from multiple complications, including urinary tract infection (UTI) and nephropathy were investigated. Antibiogram assays of the bacterial isolates from collected samples demonstrated resistance against most of the antibiotics tested. Further studies were conducted to determine the types of resistant bacteria that caused UTI. Analyzing the 16S rDNA sequence and phylogenetic tree, 3 isolates were identified as <em>Escherichia coli</em>, 5 as <em>Klebsiella pneumoniae </em>and the rest 2 as <em>Enterobacter asburiae</em>. The findings of this research indicate the necessity of urgent attention to find an effective alternative drug for treating infections caused by these resistant isolates.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 87-93, 2021 (June)</p> 2021-06-14T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/54037 Pharmacological Screening of Substituted Benzimidazole Derivatives 2021-06-20T02:05:53+00:00 Poushali Saha smarahman@du.ac.bd Shejuti Rahman Brishty smarahman@du.ac.bd SM Abdur Rahman smarahman@du.ac.bd <p>In the present study some substituted benzimidazole derivatives were screened for several biological activities. The synthesized compounds were subjected to evaluation of central analgesic, anti-inflammatory, cytotoxicity, antimicrobial and antioxidant activities by radiant heat induced tail flicking, carrageenan induced rat paw edema inhibition, brine shrimp lethality bioassay, disc diffusion and DPPH free radical scavenging methods, respectively. Compounds <strong>2a, 2c </strong>and <strong>2d </strong>elongated the tail flicking time by 58.07-, 51.59- and 76.65%, respectively (p &lt; 0.001) at 50 mg/kg body weight dose compared to the standard morphine (87.17%). Compounds <strong>2b, 2c </strong>and <strong>2d </strong>showed prominent anti-inflammatory activity at 100 mg/kg body weight dose (% of paw edema inhibition 81.75%, 79.09% and 86.69%, respectively) compared to the standard aceclofenac (87.83%). Among the synthesized benzimidazole derivatives, compounds <strong>1a, 1b, 1c, 2a </strong>and <strong>2d </strong>exhibited potent cytotoxicity with the IC50 values of 5.47-, 11.92-, 4.55-, 7.63- and 7.94 μg/ml, respectively. In addition, compounds <strong>1c </strong>and <strong>2d </strong>displayed mild to moderate zone of inhibition <strong>(8-12 mm). </strong>On the other hand, <strong>1a </strong>and <strong>1b </strong>showed very mild antioxidant activity with IC50 values of 12.25 × 103 μg/ml and 87.33 ×103 μg/ml. Among all the derivatives, <strong>2c, 2d </strong>and <strong>1c </strong>can be potential candidates for designing new analgesic, anti-inflammatory and anti-cancer agents in future.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 95-102, 2021 (June)</p> 2021-06-14T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/54038 Phytochemical Analysis, Antioxidant and Cytotoxic Activities of Mucuna pruriens Leaves 2021-06-20T02:05:54+00:00 Shifat E Ferdous muhit@du.ac.bd Mamunur Rahman muhit@du.ac.bd Firoj Ahmed muhit@du.ac.bd Md Abdul Muhit muhit@du.ac.bd <p><em>Mucuna pruriens </em>is a tropical legume native to Africa, India and Bangladesh and is widely cultivated in tropical countries. In this study, a crude methanolic extract of the leaves of <em>M. pruriens </em>was investigated for its chemical constituents and to explore the phenolic and flavonoid content, antioxidant, cytotoxic and antimicrobial activities using established protocols. From the ethyl acetate soluble fraction of the crude methanol extract, three known compounds namely ferulic acid (<strong>1</strong>), 2-(5-methoxy-1-benzofuran-3-yl)-<em>N</em>-ethylethanamine (<strong>2</strong>) and stizolamine (<strong>3</strong>) were isolated and their structures were elucidated by the analysis of NMR spectral data. The crude extract was found to possess phenolic content of 216.16 μg/g whereas the concentration of flavonoid was found to 214.8 μg/g expressed in quercetin standard. Free radicals generated through DPPH were neutralized by crude methanolic extract and the IC50 value was obtained as 19.63 μg/ml. Regression analysis during brine shrimp lethality test enumerated LC50 value of crude methanolic extract at 10.72 μg/ml and was significant compared to the positive standard. The crude methanolic extract of leaf of <em>M. pruriens </em>did not show any significant antimicrobial activity against the organisms used in our test.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 103-109, 2021 (June)</p> 2021-06-14T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/54039 In vitro and in vivo Evaluation of Pharmacological Potential of Lasia spinosa Linn. 2021-06-20T02:05:55+00:00 Piyali Chowdhury r.pchem@yahoo.com - Mohammed Ibrahim r.pchem@yahoo.com Sarrin Shahadat r.pchem@yahoo.com Md Ruhul Kuddus r.pchem@yahoo.com Mohammad A Rashid r.pchem@yahoo.com <p><em>Lasia spinosa </em>Linn<em>. </em>(Family: Araceae) is an important medicinal plant, which is traditionally used for treatment of different human ailments. The present study was undertaken to evaluate the <em>in vitro </em>thrombolytic, antiinflammatory and <em>in vivo </em>analgesic and hypoglycemic potentials of <em>n</em>-hexane, chloroform and aqueous soluble fractions of methanol extract of <em>L. spinosa </em>whole plant. Additionally, phytochemical screening was carried out by qualitative tests, which confirmed the presence of alkaloids, glycosides, steroids, tannins, saponin in this plant. During <em>in vitro </em>thrombolytic assay, the aqueous fraction at a dose of 500 μg/100 μl showed the maximum 33.15% lysis of the blood clot, as compared to the standard streptokinase (80.10%). The <em>in vitro </em>anti-inflammatory test was performed by inhibition of egg albumin denaturation assay and RBC membrane stabilization method. The chloroform fraction exhibited maximum anti-inflammatory potential by inhibiting 51.53% denaturation of albumin and by inhibiting 54.8% hemolysis of RBC membrane against hypotonic solution. Analgesic activity was evaluated by tail immersion method for central mechanism and by formalin-induced lick test for peripheral mechanism in mice. In tail immersion method, all the solvent fractions of <em>L. spinosa </em>at a dose of 500 mg/kg body weight exhibited a significant (p&lt;0.05) elongation in pain reaction time. In peripheral analgesic activity test, the chloroform fraction at a dose of 500 mg/kg body weight inhibited a maximum of 35.44% licking response induced by formalin, as compared to the standard aspirin (53.22%). In the hypoglycemic activity test, all the fractions showed a moderate effect in reducing the blood glucose level in mice treated with 10% glucose. In conclusion, the plant <em>L. spinosa </em>can be considered as a promising source of bioactive compounds for the development of new phytomedicine.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 111-119, 2021 (June)</p> 2021-06-14T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/54040 Development and Characterization of a Combination Tablet Dosage Form Containing Sofosbuvir and Ribavirin Using Design of Experiments (DoE) Approach 2021-06-20T02:05:57+00:00 Md Anisul Islam rouf321@yahoo.com Md Mahbubul Alam rouf321@yahoo.com KM Yasif Kayes Sikdar rouf321@yahoo.com ASM Monjur Al Hossain rouf321@yahoo.com Abu Shara Shamsur Rouf rouf321@yahoo.com <p>Worldwide more than 180 million people get infected by chronic Hepatitis C Virus (HCV) and around 700,000 people pass away every year due to HCV related problems. Sofosbuvir and Ribavirin are prescribed as combined medication for the management of HCV infection globally. Therefore, optimization of a pharmaceutical dosage form containing Sofosbuvir and Ribavirin can open a new hope in the treatment of HCV infection. This study was conducted by using the Design of Experiments (DoE) approach. It is a systematic process aiming to determine the correlation between formulation factors affecting the approach and the output of the approach. During the development of formulations, two factors were considered, which are Croscarmellose Sodium (CCS) and Povidone K30 (PK30) at different concentrations ranging from 0.19% to 2.31% of the total tablet weight in 9 different formulations. Their effect on disintegration time (DT) was evaluated through statistical method and it was found between 3±0.003 and 6.5±0.015 minutes. The use of these two different disintegrants exhibited a significant effect on DT. The contour plot showed predicted ranges of concentrations of CCS and PK30 for desired DT. Fourier Transform Infrared Spectroscopy (FTIR) data, Thermogravimetric Analysis (TGA) and X-ray Diffractometry (XRD) spectrums confirmed that there was no interaction between Sofosbuvir and Ribavirin or with any other excipients used in this experiment.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 121-133, 2021 (June)</p> 2021-06-14T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences https://www.banglajol.info/index.php/JPharma/article/view/54041 Biological Investigations of the Whole Plant of Argemone mexicana (L.) 2021-06-20T02:05:36+00:00 Farjana Rahman Chaity msr@du.ac.bd Mohammad A Rashid msr@du.ac.bd Mohammad Sharifur Rahman msr@du.ac.bd <p>Abstract not available</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 135-138, 2021 (June)</p> 2021-06-14T00:00:00+00:00 Copyright (c) 2021 Dhaka University Journal of Pharmaceutical Sciences