@article{Chowdhury_Mohammed Ibrahim_Shahadat_Kuddus_Rashid_2021, title={In vitro and in vivo Evaluation of Pharmacological Potential of Lasia spinosa Linn.}, volume={20}, url={https://www.banglajol.info/index.php/JPharma/article/view/54039}, DOI={10.3329/dujps.v20i1.54039}, abstractNote={<p><em>Lasia spinosa </em>Linn<em>. </em>(Family: Araceae) is an important medicinal plant, which is traditionally used for treatment of different human ailments. The present study was undertaken to evaluate the <em>in vitro </em>thrombolytic, antiinflammatory and <em>in vivo </em>analgesic and hypoglycemic potentials of <em>n</em>-hexane, chloroform and aqueous soluble fractions of methanol extract of <em>L. spinosa </em>whole plant. Additionally, phytochemical screening was carried out by qualitative tests, which confirmed the presence of alkaloids, glycosides, steroids, tannins, saponin in this plant. During <em>in vitro </em>thrombolytic assay, the aqueous fraction at a dose of 500 μg/100 μl showed the maximum 33.15% lysis of the blood clot, as compared to the standard streptokinase (80.10%). The <em>in vitro </em>anti-inflammatory test was performed by inhibition of egg albumin denaturation assay and RBC membrane stabilization method. The chloroform fraction exhibited maximum anti-inflammatory potential by inhibiting 51.53% denaturation of albumin and by inhibiting 54.8% hemolysis of RBC membrane against hypotonic solution. Analgesic activity was evaluated by tail immersion method for central mechanism and by formalin-induced lick test for peripheral mechanism in mice. In tail immersion method, all the solvent fractions of <em>L. spinosa </em>at a dose of 500 mg/kg body weight exhibited a significant (p<0.05) elongation in pain reaction time. In peripheral analgesic activity test, the chloroform fraction at a dose of 500 mg/kg body weight inhibited a maximum of 35.44% licking response induced by formalin, as compared to the standard aspirin (53.22%). In the hypoglycemic activity test, all the fractions showed a moderate effect in reducing the blood glucose level in mice treated with 10% glucose. In conclusion, the plant <em>L. spinosa </em>can be considered as a promising source of bioactive compounds for the development of new phytomedicine.</p> <p>Dhaka Univ. J. Pharm. Sci. 20(1): 111-119, 2021 (June)</p>}, number={1}, journal={Dhaka University Journal of Pharmaceutical Sciences}, author={Chowdhury, Piyali and Mohammed Ibrahim, - and Shahadat, Sarrin and Kuddus, Md Ruhul and Rashid, Mohammad A}, year={2021}, month={Jun.}, pages={111–119} }