Effect of Calcium Carbide on Rat Tissue

Authors

  • Younus Patoare Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka-125, Bangladesh
  • Md Iqbal Hossain Department of Clinical Pharmacy & Pharmacology, Dhaka University, Dhaka-1000, Bangladesh
  • Mohammad Nurul Islam Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh
  • Akhtarunnessa Chowdhury Department of Zoology, University of Dhaka, Dhaka-1000, Bangladesh
  • Seheli Parveen Department of Clinical Pharmacy & Pharmacology, Dhaka University, Dhaka-1000, Bangladesh
  • Md Mahboob Hossain Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka-125, Bangladesh
  • Abul Hasnat Department of Clinical Pharmacy & Pharmacology, Dhaka University, Dhaka-1000, Bangladesh

DOI:

https://doi.org/10.3329/dujps.v6i2.682

Keywords:

Calcium carbide, Histopathological study, Isozymes

Abstract

To evaluate the effects of Calcium carbide (CaC2) in biological system, an in vivo study was carried out on Long Evans rats. CaC2 was administered orally once daily for one month with specific concentrations. Group- I was considered as the control group (without CaC2), Group - II, III, IV and V were the sample groups treated with CaC2 having concentration of 1g/kg, 2g/kg, 5g/kg and 10g/kg body weight respectively. The experiment was conducted to detect any cellular and molecular level changes caused by CaC2. The histopathology and isozyme assay were performed to analyze the changes in the activities of the genes affected by the free radicals released from CaC2. The molecular analysis included different isozymes namely esterase and acid phosphatase. Polyacrylamide electrophoresis of whole cell extract of control subjects and CaC2 administered rats were performed; subsequently the gels were treated with the substrates for acid phosphatase and esterase respectively. No difference was observed in the whole cell extract band pattern between the control subject and the CaC2 administered rats, which grossly indicates that the CaC2 has no effect on the expression pattern of isozymes (acid phosphatase and esterase). Histopathological analysis of liver, heart, spleen, kidney and lungs were performed to observe any change due to the administration of CaC2. Remarkable changes were observed during the histopathological study of lungs and kidney only. The histopathological analysis of kidney showed the thickening of the lining of collecting tubules with changes in cell structure while lungs were found to be increased moderately in weight, with focal areas of consolidation that was found red-brown to red. Key words: Calcium carbide; Histopathological study; Isozymes Dhaka Univ. J. Pharm. Sci. 6(2): 93-98, 2007 (December)

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Author Biography

Abul Hasnat, Department of Clinical Pharmacy & Pharmacology, Dhaka University, Dhaka-1000, Bangladesh

Correspondence to: Abul Hasnat Email- ahasnat99@yahoo.com Tel. 02-8612069; Fax-02-8615583

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How to Cite

Patoare, Y., Hossain, M. I., Islam, M. N., Chowdhury, A., Parveen, S., Hossain, M. M., & Hasnat, A. (2008). Effect of Calcium Carbide on Rat Tissue. Dhaka University Journal of Pharmaceutical Sciences, 6(2), 93–98. https://doi.org/10.3329/dujps.v6i2.682

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