In vitro Comparative Studies of the Leaf Extracts of Four Different Types of Calamus Species

Authors

  • Munsifa Sultana Phytochemistry Research Laboratory, Department of Pharmacy, University of Asia Pacific 74/A, Green Road, Farmgate, Dhaka-1205, Bangladesh
  • Sajan Das Phytochemistry Research Laboratory, Department of Pharmacy, University of Asia Pacific 74/A, Green Road, Farmgate, Dhaka-1205, Bangladesh
  • Mohammad Shahriar Phytochemistry Research Laboratory, Department of Pharmacy, University of Asia Pacific 74/A, Green Road, Farmgate, Dhaka-1205, Bangladesh
  • Rumana Akhter Phytochemistry Research Laboratory, Department of Pharmacy, University of Asia Pacific 74/A, Green Road, Farmgate, Dhaka-1205, Bangladesh
  • Isa Naina Mohamed Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur 56000, Malaysia
  • Mohiuddin Ahmed Bhuiyan Phytochemistry Research Laboratory, Department of Pharmacy, University of Asia Pacific 74/A, Green Road, Farmgate, Dhaka-1205, Bangladesh

DOI:

https://doi.org/10.3329/dujps.v21i2.63118

Keywords:

Thrombolytic activity, membrane stabilizing activity, antioxidant profile, Calamus, membrane stabilizing

Abstract

The leaf extracts of Calamus tenius, Calamus viminalis, Calamus guruba and Calamus erectus possessed various phytochemicals including alkaloids, carbohydrates, saponins, phenols, tannins, flavonoids, glycosides, fixed oils and steroids. The point of the present investigation was to compare the antioxidant profile of different leaf extracts of these species. In the present study, methanol, ethanol and chloroform extract of leaf of C. tenius, C. viminalis, C. guruba and C. erectus were explored for in-vitro thrombolytic activity, membrane stabilizing activity by using hypotonic solution-induced & heat-induced hemolysis and antioxidant profile. Ethanol extracts of all plant species demonstrated the highest percentage of clot lysis. During hypotonic solution-induced hemolysis, methanol extract of C. erectus inhibited 59.74% whereas during heat induced condition ethanol extracts of all plant species demonstrated the highest percentage inhibition of hemolysis of RBCs. The screenings of the plants revealed that leaf extracts contain phenolic and flavonoid contents in great amount with the high level of antioxidant capacity. The chloroform extract of C. tenius (2.45 μg/ml) and methanol extract of C. erectus (1.74 μg/ml) leaf showed potent DPPH and H2O2 scavenging activity, respectively. The nitric oxide scavenging activity was not significantly different for methanol, ethanol and chloroform extracts. Methanol, ethanol and chloroform extracts of these four plants showed medium to moderate reducing power and cupric reducing capacity in comparison to standard. A strong relationship was found between the antioxidant activity of the extracts with their total phenolic and flavonoid contents.

Dhaka Univ. J. Pharm. Sci. 21(2): 173-181, 2022 (December)

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Published

2022-12-28

How to Cite

Sultana, M. ., Das, S. ., Shahriar, M. ., Akhter, R. ., Mohamed, I. N. ., & Bhuiyan, M. A. . (2022). In vitro Comparative Studies of the Leaf Extracts of Four Different Types of Calamus Species. Dhaka University Journal of Pharmaceutical Sciences, 21(2), 173–181. https://doi.org/10.3329/dujps.v21i2.63118

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