Antidiabetic Activity of Momordica charantia L. and Mechanism of Insulin Secretion of 1-Butanol Soluble Part on Type 2 Diabetic Model Rats

Authors

  • M Shakhawoat Hossain Department of Arts and Sciences, Ahsanullah University of Science and Technology 141-142, Love Road, Tejgaon Industrial Area, Dhaka-1208, Bangladesh
  • MK Hasan Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh
  • M Shoeb Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh
  • M I R Mamun Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh
  • N Nahar Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh
  • L Ali Bangladesh University of Health Sciences, 125/1, Darus Salam, Mirpur, Dhaka 1216, Bangladesh
  • M Mosihuzzaman Bangladesh University of Health Sciences, 125/1, Darus Salam, Mirpur, Dhaka 1216, Bangladesh
  • B Rokeya Bangladesh University of Health Sciences, 125/1, Darus Salam, Mirpur, Dhaka 1216, Bangladesh
  • HS Chaudhury Department of Biochemistry, International Medical College and Hospital, Gushulia, Sataish, Tongi, Gazipur, Bangladesh

DOI:

https://doi.org/10.3329/dujps.v19i2.50625

Keywords:

Momordica charantia, antidiabetic effect, insulin secretion, pancreatic perfusion

Abstract

The in vivo effects of fruit pulp juice (MC-PJ) of Momordica charantia and its 1-butanol soluble part (MC-BP) and aqueous soluble part (MC-AP) on blood glucose of type 2 diabetic rats were studied. In vitro insulin secretion in response to MC-BP and MC-AP from whole perfused pancreas was measured. For elucidating the mechanism of insulinotropic action, the insulin secretory activity of MC-BP in the presence of 11 mM glucose, 50 μM verapamil (Ca++ channel blocker), 8 mM diazoxide (K+ ATP channel opener) and 10 mM theophylline (cAMP phosphodiesterase inhibitor) were studied. Serum glucose was measured by glucose oxidase-peroxidase method and rat insulin was assayed by specific ELISA. In the in vivo study, MC-BP significantly opposed the rise of serum glucose compared to control at 105 min (p<0.05). Although the MC-AP and MC-PJ lowered the serum glucose both at 60 and 105 min, these were not statistically significant. In the in vitro study, only MC-BP produced 22-fold increase in insulin secretion from the perfused pancreas at nonstimulatory glucose level, which was significant (basal vs. MC-BP, 0.071±0.009 vs. 1.563±0.150 ng/ml, p<0.001). The MC-BP also enhanced the insulin secretion from the glucose-stimulated pancreas (p<0.001). The MC-BP induced insulin secretion was not affected in presence of diazoxide and verapamil. The obtained results also showed that MC-BP enhanced the insulin secretory effect of theophylline (p<0.001). The findings indicate that MC-BP has stimulatory effects on physiological pathways of insulin secretion which may underlie its reported antidiabetic action.

Dhaka Univ. J. Pharm. Sci. 19(2): 111-117, 2020 (December)

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Published

2020-12-10

How to Cite

Hossain, M. S., Hasan, M., Shoeb, M., Mamun, M. I. R., Nahar, N., Ali, L., Mosihuzzaman, M., Rokeya, B., & Chaudhury, H. (2020). Antidiabetic Activity of Momordica charantia L. and Mechanism of Insulin Secretion of 1-Butanol Soluble Part on Type 2 Diabetic Model Rats. Dhaka University Journal of Pharmaceutical Sciences, 19(2), 111–117. https://doi.org/10.3329/dujps.v19i2.50625

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