HPLC-UV Determination of Catechins and Gallic Acid in Aerial Parts of Astragalus glycyphyllos L.

  • Lysiuk Roman Department of Pharmacognosy and Botany, Danylo Halytsky Lviv National Medical University Pekarska Str., 69, Lviv, 79010, Ukraine
  • Kozachok Solomiia Department of Pharmacognosy and Medical Botany, I.Horbachevsky Ternopil State Medical University Ruska Str., 36, Ternopil, 46002, Ukraine
Keywords: Astragalus glycyphyllos, HPLC-UV, nephroprotectors, catechins, gallic acid

Abstract

The aerial part of Astragalus glycyphyllos L. (Astragali glycyphylli herba), a promising nephroprotective agent in folk medicine, has been investigated by HPLC-UV for occurrence of catechins, which are known in individual state as renoprotective substances and gallic acid. The following six compounds were detected and quantitatively determined in the analyzed plant material: (-)-gallocatechin, (-)-epigallocatechin, (+)-catechin, (-)-epicatechin, (-)-epicatechin gallate and gallic acid. The highest yield amongst the flavan-3-ols was found for (-)-gallocatechin (3.7 mg/g) and (-)-epigallocatechin (1.7 mg/g). The determined content of gallic acid was 0.4 mg/g. The investigated material should be subjected for further studies to substantiate the recommendation for its wider employment in current phytotherapeutic practice.

Dhaka Univ. J. Pharm. Sci. 18(2): 241-247, 2019 (December)

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Abstract
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Published
2019-12-12
How to Cite
Roman, L., & Solomiia, K. (2019). HPLC-UV Determination of Catechins and Gallic Acid in Aerial Parts of Astragalus glycyphyllos L. Dhaka University Journal of Pharmaceutical Sciences, 18(2), 241-247. https://doi.org/10.3329/dujps.v18i2.44464
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