Alternanthera bicolor Produces Hypoglycemic Effect in Alloxan-Induced Diabetic Mice through its Antioxidant Activity

  • Kousik Ahmed Khan Department of Pharmacy, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka-1217, Bangladesh
  • Manik Zahan Department of Pharmacy, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka-1217, Bangladesh
  • Fatema Zohura Talukder Department of Pharmacy, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka-1217, Bangladesh
  • Riaz Uddin Department of Pharmacy, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka-1217, Bangladesh
  • Manik Chandra Shill Department of Pharmaceutical Sciences, North South University, Basundhara, Dhaka 1229, Bangladesh
  • Hemayet Hossain BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR) Dhaka-1205, Bangladesh
  • Lutfun Nahar School of Pharmacy and Biomolecular Sciences, Faculty of Sciences, Liverpool John Moores University James Parsons Building, Byrom Street, Liverpool LV3 3AF, England, UK
  • Satyajit Dey Sarker School of Pharmacy and Biomolecular Sciences, Faculty of Sciences, Liverpool John Moores University James Parsons Building, Byrom Street, Liverpool LV3 3AF, England, UK
  • Md Ashraful Alam Department of Pharmaceutical Sciences, North South University, Basundhara, Dhaka 1229, Bangladesh
  • Hasan Mahmud Reza Department of Pharmaceutical Sciences, North South University, Basundhara, Dhaka 1229, Bangladesh
Keywords: A. bicolor, lipid peroxidation, caffeic acid, quercetin, diabetes

Abstract

The current investigation was carried out to evaluate the antioxidant properties of ethanolic extract of Alternanthera bicolor and to assess the potential hypoglycemic effect of the extract in alloxan-induced diabetic mice. HPLC-DAD method was used to determine polyphenolic compounds present in the extract. Different in vitro assays (i.e. DPPH radical scavenging activity test, reducing power test, NO radical inhibition assay and scavenging of hydrogen peroxide) were used to determine the antioxidant potential of the plant. Antidiabetic activity was evaluated in alloxan-induced diabetic mice by glucose tolerance test and standard biochemical analyses. HPLC-DAD analysis of the extract confirmed the presence of (+)-catechin hydrate, caffeic acid, quercetin and kaempferol. A. bicolor showed potent antioxidant activities in DPPH radical, hydrogen peroxide and nitric oxide scavenging assays. Moreover, A. bicolor showed potent reducing power and dose-dependent increment of total antioxidant capacity. Furthermore, the plant showed potent hypoglycemic activities in alloxan-induced diabetic mice. Ethanolic extract of the plant at doses of 200- and 400-mg/kg body weight (administered orally for 3 weeks) significantly decreased the elevated levels of blood glucose, lipid peroxidation product TBARS, hydroperoxides and nitric oxide in experimental animals. Apart from these activities, the ethanolic extract of the plant restored the reduced catalase function in liver. From this study, we can conclude that ethanolic extract of A. bicolor exhibited hypoglycemic and antioxidant activities in alloxan-induced diabetic mice.

Dhaka Univ. J. Pharm. Sci. 18(1): 49-60, 2019 (June)

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Abstract
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Published
2019-05-16
How to Cite
Khan, K., Zahan, M., Talukder, F., Uddin, R., Shill, M., Hossain, H., Nahar, L., Sarker, S., Alam, M. A., & Reza, H. (2019). Alternanthera bicolor Produces Hypoglycemic Effect in Alloxan-Induced Diabetic Mice through its Antioxidant Activity. Dhaka University Journal of Pharmaceutical Sciences, 18(1), 49-60. https://doi.org/10.3329/dujps.v18i1.41431
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