Simultaneous Determination of Caffeine, Preservatives and Antioxidants in Energy- and Soft-Drinks Commercially Available in Bangladesh

Authors

  • Md Samiul Islam Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • Md Shahadat Hossain Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • Subrata Bhadra Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • Abu Shara Shamsur Rouf Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000

DOI:

https://doi.org/10.3329/dujps.v15i1.29203

Keywords:

HPLC, Caffeine, Benzoic acid, Parabens, Energy drinks, Soft drinks, USFDA, JECFA

Abstract

This study describes a validated assay method to determine caffeine, benzoic acid, methylparaben, propylparaben, butylparaben, butylatedhydroxyanisole, and butylatedhydroxytoluene simultaneously by reversed phase high performance liquid chromatographic (HPLC) method. The separation of compounds was achieved on a C18 column in a gradient of acetonitrile and diluted sulfuric acid (pH=2.3) and quantification was performed by using a UV-detector set at 265 nm. The method was found to be linear over the concentration range of 60-90 ppm (R2=0.995), 200-300 ppm (R2=0.993), 16-24 ppm (R2=0.990), 16-24 ppm (R2=0.994), 16-24 ppm (R2=0.997), 48-72 ppm (R2=0.993), and 32-48 ppm (R2=0.994) for caffeine, benzoic acid, methylparaben, propylparaben, butylparaben, butylatedhydroxyanisole, and butylatedhydroxytoluene, respectively. Sixty energy- and soft-drink samples were analyzed where about 53% were found to contain caffeine among which 16% exceeded the tolerance limit (>200 ppm) set by USFDA. Moreover, 65% of analyzed samples contained benzoic acid and 10% of those samples exceeded the maximum allowable limit (>600 ppm) set by joint expert committee for food additives (JECFA).

Dhaka Univ. J. Pharm. Sci. 15(1): 97-108, 2016 (June)

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Author Biography

Md Samiul Islam, Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000



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Published

2016-08-08

How to Cite

Islam, M. S., Hossain, M. S., Bhadra, S., & Rouf, A. S. S. (2016). Simultaneous Determination of Caffeine, Preservatives and Antioxidants in Energy- and Soft-Drinks Commercially Available in Bangladesh. Dhaka University Journal of Pharmaceutical Sciences, 15(1), 97–108. https://doi.org/10.3329/dujps.v15i1.29203

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Articles