Interaction of Duloxetine Hydrochloride with Deoxyribonucleic Acid Measured by Fluorescence Spectroscopy

Authors

  • Raznin Akter Joly Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy University of Dhaka, Dhaka-1000
  • Md Reazul Islam Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy University of Dhaka, Dhaka-1000
  • Sonia Sultana Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy University of Dhaka, Dhaka-1000
  • Asma Rahman Centre for Advanced Research in Sciences, University of Dhaka, Dhaka-1000
  • Md Zakir Sultan Centre for Advanced Research in Sciences, University of Dhaka, Dhaka-1000
  • Mohammad Safiqul Islam Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhai-3814
  • Md Saiful Islam Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy University of Dhaka, Dhaka-1000
  • Abul Hasnat Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy University of Dhaka, Dhaka-1000

DOI:

https://doi.org/10.3329/dujps.v14i2.28511

Keywords:

Calf thymus DNA, affinity, stern volmer constant, fluorescence quenching, hypochromic effect, minor groove

Abstract

Interactions with many clinically active therapeutic agents with DNA are well studied and it is necessary to decipher the structure of DNA and to investigate the pathological implications of those molecules in living organism. This study investigated the interaction of antidepressant drug Duloxetine-hydrochloride (DLX) with calf thymus DNA (ct-DNA). The interaction of DLX with ct-DNA was studied employing fluorescence spectroscopy. Hypochromic effect was found in the absorption spectra of duloxetine, and its wavelength had no shift in the presence of DNA indicating external binding mode of duloxetine to DNA. Fluorescence spectroscopic results showed the quenching of fluorescence intensity of DLX in presence of DNA indicating the interaction between DLX and DNA. Hydrophobic interaction and hydrogen bonding played the dominating role in DLX-DNA binding and binding forces also indicate the binding site of duloxetine to be at the minor groove of DNA.

Dhaka Univ. J. Pharm. Sci. 14(2): 199-206, 2015 (December)

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Author Biography

Raznin Akter Joly, Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy University of Dhaka, Dhaka-1000



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Published

2016-06-28

How to Cite

Joly, R. A., Islam, M. R., Sultana, S., Rahman, A., Sultan, M. Z., Islam, M. S., Islam, M. S., & Hasnat, A. (2016). Interaction of Duloxetine Hydrochloride with Deoxyribonucleic Acid Measured by Fluorescence Spectroscopy. Dhaka University Journal of Pharmaceutical Sciences, 14(2), 199–206. https://doi.org/10.3329/dujps.v14i2.28511

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