Development and Validation of Bioanalytical HPLC Method For Estimation of Telmisartan In Rat Plasma: Application To Pharmacokinetic Studies

Authors

  • Jaydeep M Patel Department of Pharmaceutical Sciences, Saurashtra University, Rajkot-360005, Gujarat
  • Anjali P Dhingani Atmiya Institute of Pharmacy, Kalawad Road, Rajkot-360005, Gujarat
  • Kevin C Garala Atmiya Institute of Pharmacy, Kalawad Road, Rajkot-360005, Gujarat
  • Mihir K Raval Department of Pharmaceutical Sciences, Saurashtra University, Rajkot-360005, Gujarat
  • Navin R Sheth Department of Pharmaceutical Sciences, Saurashtra University, Rajkot-360005, Gujarat

DOI:

https://doi.org/10.3329/dujps.v11i2.14562

Keywords:

Bioanalytical method, Telmisartan, HPLC, Pharmacokinetics, Rat plasma

Abstract

A simple, specific, sensitive and rapid reversed phase high performance liquid chromatographic (HPLC) method has been developed and validated for the determination of telmisartan in small volumes of rat plasma. Biological sample preparation involving simple extraction with organic solvent, followed by dilution with mobile phase was adopted to eliminate any chromatographic solvent effects. The method was proven to be linear over a plasma concentration range of 10 to 1000 ng/mL with a mean correlation coefficient of 0.9942. The limit of detection and the limit of quantification of the newly developed method were determined to be 1 ng/mL and 10 ng/mL, respectively. The method was successfully applied to assess pharmacokinetic parameters of telmisartan in Wister rats following a single oral dose (1.8 mg/kg, b.w.). The developed method was established as a rapid analytical tool in a pharmacokinetic study as it required short retention time, high precision, sensitivity and small volumes of plasma for analysis.

DOI: http://dx.doi.org/10.3329/dujps.v11i2.14562

Dhaka Univ. J. Pharm. Sci. 11(2): 121-127, 2012 (December)

Downloads

Download data is not yet available.
Abstract
3509
PDF
3489

Downloads

Published

2013-04-14

How to Cite

Patel, J. M., Dhingani, A. P., Garala, K. C., Raval, M. K., & Sheth, N. R. (2013). Development and Validation of Bioanalytical HPLC Method For Estimation of Telmisartan In Rat Plasma: Application To Pharmacokinetic Studies. Dhaka University Journal of Pharmaceutical Sciences, 11(2), 121–127. https://doi.org/10.3329/dujps.v11i2.14562

Issue

Section

Articles