Spectroscopic Studies of the Interaction between Metformin Hydrochloride and Bovine Serum Albumin

  • Ahmad Tanwir Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000
  • Rahat Jahan Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka 1000
  • Mohiuddin Abdul Quadir Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000
  • Mohammad A Kaisar Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000
  • Md Khalid Hossain Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000
Keywords: Metformin hydrochloride, bovine serum albumin, fluorescence quenching, thermodynamic parameters

Abstract

The affinity of a drug to serum albumin has influence on the pharmacokinetics of a drug. In the present study, the mutual interaction of metformin hydrochloride (MET) with bovine serum albumin (BSA) was investigated using fluorescence spectroscopy under different conditions. It was observed that the fluorescence  quenching of BSA by metformin hydrochloride is a result of the formation of metformin hydrochloride- BSA complex with probable involvement of tryptophan residue. Fluorescence quenching constants were determined using  the Stern- Volmer equation and Vant Hoff equation to provide a measure of the thermodynamic parameters ?G, ?H, and ?S at different temperatures indicating that the hydrogen bond and the hydrophobic forces play a major role for metformin hydrochloride- BSA association.

DOI: http://dx.doi.org/10.3329/dujps.v11i1.12486

Dhaka Univ. J. Pharm. Sci. 11(1): 45-49, 2012 (June)

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Abstract
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Published
2012-11-04
How to Cite
Tanwir, A., Jahan, R., Quadir, M., Kaisar, M., & Hossain, M. (2012). Spectroscopic Studies of the Interaction between Metformin Hydrochloride and Bovine Serum Albumin. Dhaka University Journal of Pharmaceutical Sciences, 11(1), 45-49. https://doi.org/10.3329/dujps.v11i1.12486
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