Validation and Optimization of a Simple RP-HPLC Method for Determination of Trimetazidine in Human Serum and its Application in a Pharmacokinetic Study with Healthy Bangladeshi Male Volunteers

Authors

  • Md Mazharul Islam Chowdhury Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • Md Ashik Ullah Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • Abdullah Al Maruf Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • Mohammad Safiqul Islam Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • Maizbha Uddin Ahmed Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • Md Hasanuzzaman Shohag Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • MAK Azad Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • Abul Hasnat Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000

DOI:

https://doi.org/10.3329/dujps.v10i2.11783

Keywords:

Trimetazidine, Antianginal drug, Method development and validation, Pharmacokinetics, Bangladeshi volunteer

Abstract

Trimetazidine is an effective and well-tolerated antianginal drug. In the present study, a simple, sensitive  and specific liquid chromatography (HPLC) method with UV detection was developed and validated for the  quantification of trimetazidine in human serum samples using caffeine as internal standard. Protein precipitation  method with methanol was employed in the extraction of trimetazidine and caffeine from biological matrix. The  chromatographic separation was accomplished on Xterra C18 Column with a mobile phase consisting 0.01 M  potassium dihydrogen phosphate buffer (pH 4.16 ± 0.01 adjusted with orthophosphoric acid, with a solvent system of  triethanolamine and acetonitrile (90:10) at a flow rate of 1.0 ml/min. The chromatogram was monitored at a  wavelength of 207 nm. The method was validated over a linear concentration range of 5-200 ng/ml and limit of  quantification (LOQ) was 5.0 ng/ml with a coefficient of correlation (r2) ? 0.996. The intra-day and inter-day  precision expressed as relative standard deviation was 3.40%-11.63% and 1.30%-10.21%, respectively. The average  recovery of trimetazidine from serum was 97.44%. The method was successfully applied to a pharmacokinetic study  after oral administration of modified release trimetazidine hydrochloride tablet (35 mg) in healthy Bangladeshi  volunteers.

DOI: http://dx.doi.org/10.3329/dujps.v10i2.11783

Dhaka Univ. J. Pharm. Sci. 10(2): 71-78, 2011 (December)

 

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Published

2012-09-03

How to Cite

Chowdhury, M. M. I., Ullah, M. A., Maruf, A. A., Islam, M. S., Ahmed, M. U., Shohag, M. H., Azad, M., & Hasnat, A. (2012). Validation and Optimization of a Simple RP-HPLC Method for Determination of Trimetazidine in Human Serum and its Application in a Pharmacokinetic Study with Healthy Bangladeshi Male Volunteers. Dhaka University Journal of Pharmaceutical Sciences, 10(2), 71–78. https://doi.org/10.3329/dujps.v10i2.11783

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