Detection of Transgene in Tilapia (Oreochromis niloticus) by Polymerase Chain Reaction (PCR) Technique

  • PC Ray Department of Biotechnology, Bangladesh Agricultural University, Mymensingh- 2202
  • MS Alam Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh- 2202
  • MS Islam Department of Biotechnology, Bangladesh Agricultural University, Mymensingh- 2202
Keywords: Growth hormone (GH) gene, PCR amplification, Transgenic tilapia

Abstract

Polymerase Chain Reaction (PCR) technique using specific primer can be used to detect transgenes. The present study was undertaken to detect salmon growth hormone (GH) gene in transgenic tilapia (Oreochromis niloticus) by PCR. DNA was extracted from F1 Tilapia generated by crossing transgenic parents. Two primers were designed to amplify a part of the region of GH gene sequence, which was used to make transgenic tilapia. To confirm the specificity of the selected primer, PCR was performed on diluted DNAs, extracted from tilapia fin tissues. GH transgene sequences (1500 bp) were successfully amplified from transgenic fish in this study. The specificity of the primers was found to be high in detecting the salmon GH transgenes. The PCR-based method therefore, could be used for fast and easy screening of transgenic fish for this gene.

J. Environ. Sci. & Natural Resources, 8(2): 47-49 2015

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Author Biography

PC Ray, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh- 2202


Published
2016-02-29
How to Cite
Ray, P., Alam, M., & Islam, M. (2016). Detection of Transgene in Tilapia (Oreochromis niloticus) by Polymerase Chain Reaction (PCR) Technique. Journal of Environmental Science and Natural Resources, 8(2), 47-49. https://doi.org/10.3329/jesnr.v8i2.26864
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Articles